Sack and Yarov-Yarovoy:Protocols

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==Cell Culture==
==Cell Culture==
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12]] [http://openwetware.org/images/2/27/Cell_Media_with_Gen_Selection_Agents_4-5-2012.docx .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11]] [http://openwetware.org/images/3/3a/Cell_Media_with_Selection_Agents_11-15-2011KE.docx .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf .]<br>
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[[Sack: Cell Splitting Protocol|Cell Splitting Protocol 03/02/12]][http://openwetware.org/images/7/7f/Cell_Splitting_Protocol.doc .]<br>
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[[Sack: Cell Splitting Protocol|Cell Splitting Protocol 03/02/12]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf .]<br>
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[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX) 05/01/12]][http://openwetware.org/images/8/87/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.doc .]<br>
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[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX) 05/01/12]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf .]<br>
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[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine) 08/14/12]][http://openwetware.org/images/4/4f/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.docx .]<BR>
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[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine) 08/14/12]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR>
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[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice) 05/04/12]][http://openwetware.org/images/c/ca/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.doc .]<BR>
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[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice) 05/04/12]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf .]<BR>
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[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology 07/18/12]][http://openwetware.org/images/7/78/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.doc   .]<br>
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[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology 07/18/12]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf   .]<br>
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[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology 10/21/11]] [http://openwetware.org/images/7/76/Cell_Plating_for_Electrophysiology_SackLab.doc  .]<br>
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[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology 10/21/11]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf  .]<br>
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[[Sack: Freezing CHO cells|Freezing CHO Cells 04/05/12]][http://openwetware.org/images/b/bb/Freezing_CHOcells_SackLab.doc .]<BR>
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[[Sack: Freezing CHO cells|Freezing CHO Cells 04/05/12]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf .]<BR>
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[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys 09/29/11]][http://openwetware.org/images/f/f7/Plating_Subclones_for_Ephys_SackLab.doc .]<BR>
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[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys 09/29/11]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf .]<BR>
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[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Flourescent Screening 04/05/12]][http://openwetware.org/images/7/7e/Plating_subclones_for_fluorescent_screening_SackLab.doc .]<BR>
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[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Flourescent Screening 04/05/12]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf .]<BR>
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[[Sack: Subcloning Cells| Subcloning Cells 02/23/11]][http://openwetware.org/images/9/9d/Subcloning_Cells_SackLab_2-23-2011_KE.doc .]<BR>
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[[Sack: Subcloning Cells| Subcloning Cells 02/23/11]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf .]<BR>
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[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys 04/06/11]][http://openwetware.org/images/c/c2/Testing_TREX_Subclones_Ephys.doc .]<BR>
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[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys 04/06/11]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf .]<BR>
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[[Sack: Thawing CHOcells| Thawing CHO cells 07/09/12]][http://openwetware.org/images/8/81/Thawing_CHOcells_SackLab_7-9-2012.doc .]<BR>
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[[Sack: Thawing CHOcells| Thawing CHO cells 07/09/12]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf .]<BR>
==Flourophore Labeling==
==Flourophore Labeling==
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[[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/9/92/112211_OMIXC18_GxTX_purification.docx .]<br>  
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[[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/1/1f/112211_OMIXC18_GxTX_purification.pdf .]<br>  
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[[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]][http://openwetware.org/images/8/8b/Alyknefluor_Azide_beads_111111.doc .]<br>
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[[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]][http://openwetware.org/images/9/9b/Alyknefluor_Azide_beads_111111.pdf .]<br>
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[[Sack: Maleimide Labeling| Maleimide Labeling 11/08/11]][http://openwetware.org/images/c/c7/Maleimide_labeling_110811jon.docx .]<br>
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[[Sack: Maleimide Labeling| Maleimide Labeling 11/08/11]][http://openwetware.org/images/2/23/Maleimide_labeling_110811jon.pdf .]<br>
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[[Sack: Maleimide Labeling TAMRA| Maleimide Labeling TAMRA 10/08/12]][http://openwetware.org/images/2/2b/Maleimide_labeling_TAMRA_121411SA.docx .]<br>
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[[Sack: Maleimide Labeling TAMRA| Maleimide Labeling TAMRA 10/08/12]][http://openwetware.org/images/0/03/Maleimide_labeling_TAMRA_121411SA.pdf .]<br>
   
   
==General Lab==
==General Lab==

Revision as of 18:27, 10 October 2012

Cell Culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12 .
Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11 .
Cell Splitting Protocol 03/02/12.
Cell Transfection (Lipofectamine LTX) 05/01/12.
Cell Transfection (Lipofectamine) 08/14/12.
Cell Transfection (Novachoice) 05/04/12.
Cell Harvesting for Electrophysiology 07/18/12.
Cell Plating for Electrophysiology 10/21/11 .
Freezing CHO Cells 04/05/12.
Plating Subclones for Ephys 09/29/11.
Plating Subclones for Flourescent Screening 04/05/12.
Subcloning Cells 02/23/11.
Testing TREX Subclones Ephys 04/06/11.
Thawing CHO cells 07/09/12.

Flourophore Labeling

GxTx Purification 11/22/11.
Alyknefluor Azide Beads 11/11/11.
Maleimide Labeling 11/08/11.
Maleimide Labeling TAMRA 10/08/12.

General Lab

Acidwash 03/22/11



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