Sack: Subcloning Cells: Difference between revisions
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Besides reagents to trypsinize cells, you will need:<br> | Besides reagents to trypsinize cells, you will need:<br> | ||
In hood:<br> | In hood:<br> | ||
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Put 200 ul/well into a 96 well plate<br> | Put 200 ul/well into a 96 well plate<br> | ||
Place cells in incubator, check for colonies in a week<br> | Place cells in incubator, check for colonies in a week<br> | ||
Latest revision as of 16:12, 15 January 2013
Besides reagents to trypsinize cells, you will need: At microscope: Note: cells sink in media, so make sure to mix solution by pipetting up and down immediately before taking cells Rinse (DPBS), trypsinize, & resuspend cells in same volume media To count cells
Pipet >10 ul cells into small tube, remove from hood To dilute cells and plate into 96 well plates For 5 cells per 200 ul well, you will use a 25 cells/ml dilution: For 1 cells per 200 ul well, you will use a 5 cells/ml dilution: For 0.2 cells per 200 ul well, you will use a 1 cell/ml dilution: |