Sack: Cell Splitting Protocol: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{Template: Sack Lab}}<br> | |||
<div style="right: center; text-align: left; float:none"> | |||
Notes: Steps should be done inside of a Biological Safety Cabinet in Sterile conditions. Make sure that the area has been cleaned with 70% ethanol, the UV light is off, and that the blower has been on for at least 10 min before starting. Once finished close clean the area off with 70% ethanol, close the cabinet and turn on the UV light. | Notes: Steps should be done inside of a Biological Safety Cabinet in Sterile conditions. Make sure that the area has been cleaned with 70% ethanol, the UV light is off, and that the blower has been on for at least 10 min before starting. Once finished close clean the area off with 70% ethanol, close the cabinet and turn on the UV light. | ||
Line 17: | Line 20: | ||
*Cell Media: Ham’s – F12 from Gibco, 10% FBS, Penicillin/Streptomycin | *Cell Media: Ham’s – F12 from Gibco, 10% FBS, Penicillin/Streptomycin | ||
To make add 50 mL Media + 50 mL FBS | To make add 50 mL Media + 50 mL FBS | ||
Revision as of 16:09, 15 January 2013
Notes: Steps should be done inside of a Biological Safety Cabinet in Sterile conditions. Make sure that the area has been cleaned with 70% ethanol, the UV light is off, and that the blower has been on for at least 10 min before starting. Once finished close clean the area off with 70% ethanol, close the cabinet and turn on the UV light. Procedures (done in T25 flask):
To make add 50 mL Media + 50 mL FBS |