Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (18:09, 15 January 2013) (view source)
 
Line 1: Line 1:
 +
{{Template: Sack Lab}}<br>
 +
<div style="right: center; text-align: left; float:none">
 +
Revised by Kenneth Eum on 11-15-2011
Revised by Kenneth Eum on 11-15-2011
Line 16: Line 19:
# Add 12.5µl of zeocin (Final Concentration (25 µg/ml))<BR>
# Add 12.5µl of zeocin (Final Concentration (25 µg/ml))<BR>
# Close the conical tube tightly and store in 4°C<BR>
# Close the conical tube tightly and store in 4°C<BR>
-
 
-
 
-
*Return to [[Sack:Protocols]]
 

Current revision


Sack and Yarov-Yarovoy Labs

Department of Physiology and Membrane Biology
University of California, Davis

Home        Research        People        Contact        Protocols        Resources       



Revised by Kenneth Eum on 11-15-2011

Cell Media with Selection Agents (Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line
F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin

Blasticidin = 10mg/ml stock
Zeocin = 100mg/ml stock

  1. Obtain a 50mL conical tube and label as:
    • “Ham’s F-12 Media + 10% FBS + 1% Pen/Strep + 1ug/ml blast + 25ug/ml zeo”
    • Include date made and initials
  2. Add 44.5mL of Ham’s F-12 Media to the labeled 50mL conical tube
  3. Add 5mL of FBS
  4. Add 500µL of Pen Strep
  5. Add 5µl of blasticidin (Final Concentration (1 µg/ml))
  6. Add 12.5µl of zeocin (Final Concentration (25 µg/ml))
  7. Close the conical tube tightly and store in 4°C
Personal tools