Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin): Difference between revisions
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Cell Maintenance Media with Selection Agents (Zeocin, Blasticidin, Geneticin)<br> | Cell Maintenance Media with Selection Agents (Zeocin, Blasticidin, Geneticin)<br> | ||
F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin, 1/500 Geneticin<BR> | F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin, 1/500 Geneticin<BR> | ||
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*Add 125µl of zeocin to 50ml of Ham’s F-12 Media + 10% FBS + 1% Pen/Strep media (Final Concentration (250µg/ml))<BR> | *Add 125µl of zeocin to 50ml of Ham’s F-12 Media + 10% FBS + 1% Pen/Strep media (Final Concentration (250µg/ml))<BR> | ||
*Add 1000µl of geneticin to 50ml of Ham’s F-12 Media + 10% FBS + 1% Pen/Strep media (Final Concentration (1000µg/ml))<BR> | *Add 1000µl of geneticin to 50ml of Ham’s F-12 Media + 10% FBS + 1% Pen/Strep media (Final Concentration (1000µg/ml))<BR> | ||
Latest revision as of 16:08, 15 January 2013
Cell Maintenance Media with Selection Agents (Zeocin, Blasticidin, Geneticin) Blasticidin = 10mg/ml stock (pcDNA6)
To make selection media for establishing new cell lines, use 10x more selection agent for final concentrations of:
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