Sack: BTAA Peptide Click HPLC: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Copper-click Alkyne / Azide Condensation Reaction with peptide alkyne, azide fluor545 conditions- use HPLC protocol that worked for last GxTX TAMRA purification<br> sample prep: take 80ul...) |
No edit summary |
||
| Line 14: | Line 14: | ||
save 1 ml 350 nm fluorescent fractions in labeled 1.5ml tubes and freeze at -80C | save 1 ml 350 nm fluorescent fractions in labeled 1.5ml tubes and freeze at -80C | ||
*Return to [[Sack:Protocols]] | |||
Latest revision as of 16:53, 10 October 2012
Copper-click Alkyne / Azide Condensation Reaction with peptide alkyne, azide fluor545
conditions- use HPLC protocol that worked for last GxTX TAMRA purification
sample prep: take 80ul reaction add 20ul ACN + 0.5% TFA, spin hard inject supernatant rinse syringe with 150 ul 20% ACN
run in order:
Pra13 control
control + N3-545
stop, compare chromatograms for overlap
Pra13 + N3-545- collect fractions!
Pra17 control
Pra17 + N3-545- collect fractions!
save 1 ml 350 nm fluorescent fractions in labeled 1.5ml tubes and freeze at -80C
- Return to Sack:Protocols
