Sack and Yarov-Yarovoy:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Molecular Biology)
(Tentagel Azide Beads)
Line 41: Line 41:
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
-
 
-
==Tentagel Azide Beads==
 
-
[[Sack: Bead Cell Binding| Bead Cell Binding 05/15/12]][http://openwetware.org/images/f/f0/10mm_dish_cell_scraping_protocol.pdf  .]<br>
 
-
[[Sack: Beads Alykne Azide BTAA| Beads Alykne Azide BTTAA 05/16/12]][http://openwetware.org/images/1/17/Beads_alykne_Azide_BTTAA_gxtx_051612-1.pdf  .]<br>
 
-
*Return to [[Sack]] Homepage
 

Revision as of 15:22, 25 October 2012

Contents

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophysiology

Agar Bridge Formation.

general lab

Acidwash 03/22/11.

HPLC

BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.


Solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.


molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

Personal tools