Gerakis Yannis at 10:01, 23 January 2013

Gerakis Yannis — Wed, 23 Jan 2013 10:01:53 GMT

←Older revision		Revision as of 10:01, 23 January 2013
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	*from [http://probes.invitrogen.com/ Molecular Probes] now owned by [http://www.invitrogen.com/ Invitrogen]		*from [http://probes.invitrogen.com/ Molecular Probes] now owned by [http://www.invitrogen.com/ Invitrogen]
-	*nucleic acid dye
-	*membrane permeant
	*series of blue-, green-, orange and red-fluorescent dyes		*series of blue-, green-, orange and red-fluorescent dyes
-	*says that better results are ~~obtained~~ in ~~buffers without phosphate so no PBS?~~	+
-	*would require ~~some optimization~~	+	SYTO dyes are close to sybr green (they stain nucleic acid and are membrane permeant), but differ in some important point :
		+
		+	- SYTO dyes stain ds nucleic acid but are saturating at low concentration unlike Sybr green
		+
		+	- SYTO dyes require high-power diode to be efficiency excited (Found on Rotor-gene Q for exemple)
		+
		+	- SYTO dyes are known to be less inhibiting than sybr green in real time quantitative PCR
		+
		+	Because those dyes can easily saturate ds nucleic acid, they are very accurate for high resolution melting curve (HRM), which is used to detect single nucleotide mutation.
		+
		+	SYTO dyes are cyanine dyes. Nucleic acid interaction with those type dyes is complex and may be affected by a lot of factors : electrostatic, hydrophobic, and steric interactions. Cyanine dye binding appears to be cooperative, which it can explain their efficiency to saturate ds nucleic acid at low concentration.

Reshma P. Shetty at 17:14, 28 April 2005

Reshma P. Shetty — Thu, 28 Apr 2005 17:14:21 GMT

New page

*from [http://probes.invitrogen.com/ Molecular Probes] now owned by [http://www.invitrogen.com/ Invitrogen]
*nucleic acid dye
*membrane permeant
*series of blue-, green-, orange and red-fluorescent dyes
*says that better results are obtained in buffers without phosphate so no PBS?
*would require some optimization

SYTO - Revision history

Gerakis Yannis at 10:01, 23 January 2013

Reshma P. Shetty at 17:14, 28 April 2005