http://openwetware.org/index.php?title=SYTO&feed=atom&action=historySYTO - Revision history2013-05-21T07:02:23ZRevision history for this page on the wikiMediaWiki 1.13.2http://openwetware.org/index.php?title=SYTO&diff=669815&oldid=prevGerakis Yannis at 10:01, 23 January 20132013-01-23T10:01:53Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:01, 23 January 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*from [http://probes.invitrogen.com/ Molecular Probes] now owned by [http://www.invitrogen.com/ Invitrogen]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*from [http://probes.invitrogen.com/ Molecular Probes] now owned by [http://www.invitrogen.com/ Invitrogen]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*nucleic acid dye</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*membrane permeant</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*series of blue-, green-, orange and red-fluorescent dyes</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*series of blue-, green-, orange and red-fluorescent dyes</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*says that better results </del>are <del class="diffchange diffchange-inline">obtained </del>in <del class="diffchange diffchange-inline">buffers without phosphate so no PBS?</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*would </del>require <del class="diffchange diffchange-inline">some optimization</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">SYTO dyes </ins>are <ins class="diffchange diffchange-inline">close to sybr green (they stain nucleic acid and are membrane permeant), but differ </ins>in <ins class="diffchange diffchange-inline">some important point :</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">- SYTO dyes stain ds nucleic acid but are saturating at low concentration unlike Sybr green</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">- SYTO dyes </ins>require <ins class="diffchange diffchange-inline">high-power diode to be efficiency excited (Found on Rotor-gene Q for exemple)</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">- SYTO dyes are known to be less inhibiting than sybr green in real time quantitative PCR </ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Because those dyes can easily saturate ds nucleic acid, they are very accurate for high resolution melting curve (HRM), which is used to detect single nucleotide mutation.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">SYTO dyes are cyanine dyes. Nucleic acid interaction with those type dyes is complex and may be affected by a lot of factors : electrostatic, hydrophobic, and steric interactions. Cyanine dye binding appears to be cooperative, which it can explain their efficiency to saturate ds nucleic acid at low concentration.</ins></div></td></tr>
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</table>Gerakis Yannishttp://openwetware.org/index.php?title=SYTO&diff=11465&oldid=prevReshma P. Shetty at 17:14, 28 April 20052005-04-28T17:14:21Z<p></p>
<p><b>New page</b></p><div>*from [http://probes.invitrogen.com/ Molecular Probes] now owned by [http://www.invitrogen.com/ Invitrogen]<br />
*nucleic acid dye<br />
*membrane permeant<br />
*series of blue-, green-, orange and red-fluorescent dyes<br />
*says that better results are obtained in buffers without phosphate so no PBS?<br />
*would require some optimization</div>Reshma P. Shetty