SYBR Green II - Revision history

Reshma P. Shetty: /* Reference */

Reshma P. Shetty — Tue, 12 Dec 2006 22:11:15 GMT

Reference

←Older revision		Revision as of 22:11, 12 December 2006
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	==Reference==		==Reference==
	*[http://www.sigmaaldrich.com/sigma/datasheet/s9305dat.pdf Sigma-Aldrich technical bulletin] (source of much of the information above)		*[http://www.sigmaaldrich.com/sigma/datasheet/s9305dat.pdf Sigma-Aldrich technical bulletin] (source of much of the information above)
		+	*[http://probes.invitrogen.com/media/pis/mp07568.pdf Product Information from Molecular Probes]

	[[Category:Material]]		[[Category:Material]]

Reshma P. Shetty at 20:47, 11 December 2006

Reshma P. Shetty — Mon, 11 Dec 2006 20:47:56 GMT

←Older revision		Revision as of 20:47, 11 December 2006
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	==Reference==		==Reference==
	*[http://www.sigmaaldrich.com/sigma/datasheet/s9305dat.pdf Sigma-Aldrich technical bulletin] (source of much of the information above)		*[http://www.sigmaaldrich.com/sigma/datasheet/s9305dat.pdf Sigma-Aldrich technical bulletin] (source of much of the information above)
		+
		+	[[Category:Material]]

Reshma P. Shetty at 16:55, 5 December 2006

Reshma P. Shetty — Tue, 05 Dec 2006 16:55:30 GMT

←Older revision		Revision as of 16:55, 5 December 2006
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	*SYBR Green II stained RNA fluoresces green (maximum emission at 521 nm)		*SYBR Green II stained RNA fluoresces green (maximum emission at 521 nm)
	*tends to be used as a gel stain more often but also cited in flow cytometry		*tends to be used as a gel stain more often but also cited in flow cytometry
-	*Staining agarose/formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or	+	*Staining agarose/formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
-	subsequent hybridization in Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization	+	*SYBR Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to RNA (~0.54) than to double-stranded DNA (~0.36).
-	and hybridization buffers to remove the dye.	+
-	*SYBR Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to	+
-	RNA (~0.54) than to double-stranded DNA (~0.36).	+
	*The fluorescence quantum yield of the RNA/SYBR Green II complex is over seven times greater than that of the RNA/ethidium bromide complex (~0.07).		*The fluorescence quantum yield of the RNA/SYBR Green II complex is over seven times greater than that of the RNA/ethidium bromide complex (~0.07).

Reshma P. Shetty at 16:54, 5 December 2006

Reshma P. Shetty — Tue, 05 Dec 2006 16:54:56 GMT

←Older revision		Revision as of 16:54, 5 December 2006
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		+	==General information==
	*nucleic acid dye, better for RNA		*nucleic acid dye, better for RNA
	*membrane permeant		*membrane permeant
-	*excited by 488 laser	+	*excited by 488 laser (maximally at 497 nm with secondary excitation peak at 254nm)
-	*fluoresces green (maximum at 521 nm)	+	*SYBR Green II stained RNA fluoresces green (maximum emission at 521 nm)
	*tends to be used as a gel stain more often but also cited in flow cytometry		*tends to be used as a gel stain more often but also cited in flow cytometry
		+	*Staining agarose/formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or
		+	subsequent hybridization in Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization
		+	and hybridization buffers to remove the dye.
		+	*SYBR Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to
		+	RNA (~0.54) than to double-stranded DNA (~0.36).
		+	*The fluorescence quantum yield of the RNA/SYBR Green II complex is over seven times greater than that of the RNA/ethidium bromide complex (~0.07).
		+
		+	==Detection limits==
		+	*The detection limit is 500 pg of RNA per band in non-denaturing gels with 300 nm transillumination.
		+	*Can detect down to 100 pg with 254 nm epi-illumination).
		+	*On denaturing agarose/formaldehyde gels and polyacrylamide/urea gels, the sensitivity of SYBR Green II RNA gel stain is reduced, though still superior to that of ethidium bromide. Without any washing or destaining steps, SYBR Green II can detect as little as 1 ng RNA per band in agarose/formaldehyde gel or polyacrylamide/urea gel using 254 nm epi-illumination, and about 4 ng RNA per band using 300 nm trans illumination.
		+
		+	==Reference==
		+	*[http://www.sigmaaldrich.com/sigma/datasheet/s9305dat.pdf Sigma-Aldrich technical bulletin] (source of much of the information above)

Reshma P. Shetty at 17:16, 28 April 2005

Reshma P. Shetty — Thu, 28 Apr 2005 17:16:10 GMT

New page

*nucleic acid dye, better for RNA
*membrane permeant
*excited by 488 laser
*fluoresces green (maximum at 521 nm)
*tends to be used as a gel stain more often but also cited in flow cytometry