SYBR Gold

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==General information==
==General information==
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*Ten-fold more sensitive than ethidium bromide.
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*ten-fold more sensitive than ethidium bromide.
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*Greater dynamic range.
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*greater dynamic range.
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*Penetrates gels quickly.
+
*penetrates gels quickly.
*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
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*Stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly.  (DNA bands can come out curved).
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*stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly.  (DNA bands can come out curved).
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*Low background so no destaining is necessary.
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*low background so no destaining is necessary.
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*Can be excited at by standard transillumination at 300nm.
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*can be excited at by standard transillumination at 300nm.
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*Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
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*stained nucleic acids can be transferred to membranes for Northerns or Southerns.
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*Doesn't inhibit most enzymatic reactions except PCR.  PCR is sensitive to high dye concentrations.  Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation.
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*doesn't inhibit most enzymatic reactions except PCR.  PCR is sensitive to high dye concentrations.  Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation.
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*Very expensive
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*very expensive
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*Supplied at 10,000X concentration in anhydrous DMSO.
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*supplied at 10,000X concentration in anhydrous DMSO.
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*operates most efficiently between pH 7-8.5
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*stock solution is stable 6 months to 1 year at <= 20&deg;C
==References==
==References==
#[[Molecular Cloning]]
#[[Molecular Cloning]]
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#[http://probes.invitrogen.com/media/publications/120.pdf Technical information by Invitrogen]
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#[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen]
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#[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen]
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#[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen]

Revision as of 17:10, 5 December 2006

Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.

General information

  • ten-fold more sensitive than ethidium bromide.
  • greater dynamic range.
  • penetrates gels quickly.
  • Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
  • stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
  • low background so no destaining is necessary.
  • can be excited at by standard transillumination at 300nm.
  • stained nucleic acids can be transferred to membranes for Northerns or Southerns.
  • doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
  • very expensive
  • supplied at 10,000X concentration in anhydrous DMSO.
  • operates most efficiently between pH 7-8.5
  • stock solution is stable 6 months to 1 year at <= 20°C

References

  1. Molecular Cloning
  2. Technical information from Invitrogen
  3. SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen
  4. General SYBR stain tips and tricks from Invitrogen
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