SYBR Gold
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(Difference between revisions)
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==General information== | ==General information== | ||
| - | * | + | *ten-fold more sensitive than ethidium bromide. |
| - | * | + | *greater dynamic range. |
| - | * | + | *penetrates gels quickly. |
*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde. | *Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde. | ||
| - | * | + | *stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved). |
| - | * | + | *low background so no destaining is necessary. |
| - | * | + | *can be excited at by standard transillumination at 300nm. |
| - | * | + | *stained nucleic acids can be transferred to membranes for Northerns or Southerns. |
| - | * | + | *doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation. |
| - | * | + | *very expensive |
| - | * | + | *supplied at 10,000X concentration in anhydrous DMSO. |
| + | *operates most efficiently between pH 7-8.5 | ||
| + | *stock solution is stable 6 months to 1 year at <= 20°C | ||
==References== | ==References== | ||
#[[Molecular Cloning]] | #[[Molecular Cloning]] | ||
| - | #[http://probes.invitrogen.com/media/publications/120.pdf Technical information | + | #[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen] |
| + | #[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen] | ||
| + | #[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen] | ||
Revision as of 18:10, 5 December 2006
Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.
General information
- ten-fold more sensitive than ethidium bromide.
- greater dynamic range.
- penetrates gels quickly.
- Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
- stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
- low background so no destaining is necessary.
- can be excited at by standard transillumination at 300nm.
- stained nucleic acids can be transferred to membranes for Northerns or Southerns.
- doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
- very expensive
- supplied at 10,000X concentration in anhydrous DMSO.
- operates most efficiently between pH 7-8.5
- stock solution is stable 6 months to 1 year at <= 20°C
