SYBR Gold

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==References==
==References==
#[[Molecular Cloning]]
#[[Molecular Cloning]]
 +
#[http://probes.invitrogen.com/media/publications/120.pdf Technical information by Invitrogen]

Revision as of 14:49, 5 December 2006

Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.

General information

  • Ten-fold more sensitive than ethidium bromide.
  • Greater dynamic range.
  • Penetrates gels quickly.
  • Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
  • Stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
  • Low background so no destaining is necessary.
  • Can be excited at by standard transillumination at 300nm.
  • Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
  • Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
  • Very expensive
  • Supplied at 10,000X concentration in anhydrous DMSO.

References

  1. Molecular Cloning
  2. Technical information by Invitrogen
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