SYBR Gold

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*Can be excited at by standard transillumination at 300nm.
*Can be excited at by standard transillumination at 300nm.
*Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
*Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
-
*Doesn't inhibit most enzymatic reactions except PCR.  PCR is sensitive to high dye concentrations.  Address this issues by adjusting Mg>sup>2+</sup> concentration or remove stain via ethanol precipitation.
+
*Doesn't inhibit most enzymatic reactions except PCR.  PCR is sensitive to high dye concentrations.  Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation.
*Very expensive
*Very expensive
*Supplied at 10,000X concentration in anhydrous DMSO.
*Supplied at 10,000X concentration in anhydrous DMSO.

Revision as of 13:40, 5 December 2006

Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.

General information

  • Ten-fold more sensitive than ethidium bromide.
  • Greater dynamic range.
  • Penetrates gels quickly.
  • Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
  • Stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
  • Low background so no destaining is necessary.
  • Can be excited at by standard transillumination at 300nm.
  • Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
  • Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
  • Very expensive
  • Supplied at 10,000X concentration in anhydrous DMSO.

References

  1. Molecular Cloning
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