SYBR Gold: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 10: | Line 10: | ||
*Can be excited at by standard transillumination at 300nm. | *Can be excited at by standard transillumination at 300nm. | ||
*Stained nucleic acids can be transferred to membranes for Northerns or Southerns. | *Stained nucleic acids can be transferred to membranes for Northerns or Southerns. | ||
*Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg | *Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation. | ||
*Very expensive | *Very expensive | ||
*Supplied at 10,000X concentration in anhydrous DMSO. | *Supplied at 10,000X concentration in anhydrous DMSO. | ||
Revision as of 11:40, 5 December 2006
Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.
General information
- Ten-fold more sensitive than ethidium bromide.
- Greater dynamic range.
- Penetrates gels quickly.
- Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
- Stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
- Low background so no destaining is necessary.
- Can be excited at by standard transillumination at 300nm.
- Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
- Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
- Very expensive
- Supplied at 10,000X concentration in anhydrous DMSO.
