SSC

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Revision as of 05:29, 15 June 2009 by Jakob Suckale (talk | contribs) (20x recipe)
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SSC buffer contains sodium chloride and sodium citrate.

1x SSC buffer

  • 150mM NaCl
  • 15mM sodium citrate

20x SSC

  • 800ml dH2O
  • 175.3g NaCl (3M)
  • 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
  • adjust the pH to 7.0 with a few drops of 1M HCl
  • adjust the volume to 1L with dH2O
  • sterilize by autoclaving

using stock solution

  • 3M NaCl
  • 300 mM sodium citrate, pH 7.0 and
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