SSC

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Current revision (07:43, 15 June 2009) (view source)
(20x SSC)
 
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== 20x SSC ==
== 20x SSC ==
-
* 800ml dH2O
+
* 800ml dH2O (RNase free if required)
* 175.3g [http://en.wikipedia.org/wiki/Nacl NaCl] (3M)
* 175.3g [http://en.wikipedia.org/wiki/Nacl NaCl] (3M)
* 88.2g [http://en.wikipedia.org/wiki/Trisodium_citrate trisodium citrate] (NaCit; 300mM; e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S4641|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641])
* 88.2g [http://en.wikipedia.org/wiki/Trisodium_citrate trisodium citrate] (NaCit; 300mM; e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S4641|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641])
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=== using stock solutions ===
=== using stock solutions ===
* 3M NaCl
* 3M NaCl
-
* 300 mM sodium citrate, pH 7.0  
+
* 300 mM sodium citrate, pH 7.0
== protocols using SSC ==
== protocols using SSC ==

Current revision

SSC buffer is named after its ingredients Saline (sodium chloride) and Sodium Citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.

Contents

1x SSC buffer

  • 150mM NaCl
  • 15mM sodium citrate

20x SSC

  • 800ml dH2O (RNase free if required)
  • 175.3g NaCl (3M)
  • 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
  • adjust the pH to 7.0 with a few drops of 1M HCl
  • adjust the volume to 1L with dH2O
  • sterilize by autoclaving

using stock solutions

  • 3M NaCl
  • 300 mM sodium citrate, pH 7.0

protocols using SSC

See also

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