SSC: Difference between revisions
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SSC buffer | '''SSC buffer''' is named after its ingredients '''S'''aline (sodium chloride) and '''S'''odium '''C'''itrate. It is used as a buffer for blotting, hybridisation, and stringency washes in [[Northern]] and [[Southern blot]]s as well as DNA microarray. | ||
== 1x SSC buffer == | == 1x SSC buffer == | ||
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* 15mM sodium citrate | * 15mM sodium citrate | ||
== 20x SSC == | |||
* 800ml dH2O (RNase free if required) | |||
* 175.3g [http://en.wikipedia.org/wiki/Nacl NaCl] (3M) | |||
* 88.2g [http://en.wikipedia.org/wiki/Trisodium_citrate trisodium citrate] (NaCit; 300mM; e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S4641|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641]) | |||
* adjust the pH to 7.0 with a few drops of 1M HCl | |||
* adjust the volume to 1L with dH2O | |||
* sterilize by autoclaving | |||
== | === using stock solutions === | ||
* 3M NaCl | * 3M NaCl | ||
* 300 mM sodium citrate, pH 7.0 | * 300 mM sodium citrate, pH 7.0 | ||
== protocols using SSC == | |||
* [[Dahlquist:DNA Microarray Protocol]] | |||
* [[Endy:Northern Blot, 32P End-Labeled Probes]] | |||
* [[20.109(S07): cDNA synthesis and microarray]] | |||
* [[20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray]] | |||
== See also == | |||
* [http://en.wikipedia.org/wiki/SSC_buffer Wikipedia: SSC buffer] | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Buffers]] | |||
Latest revision as of 05:43, 15 June 2009
SSC buffer is named after its ingredients Saline (sodium chloride) and Sodium Citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.
1x SSC buffer
- 150mM NaCl
- 15mM sodium citrate
20x SSC
- 800ml dH2O (RNase free if required)
- 175.3g NaCl (3M)
- 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
- adjust the pH to 7.0 with a few drops of 1M HCl
- adjust the volume to 1L with dH2O
- sterilize by autoclaving
using stock solutions
- 3M NaCl
- 300 mM sodium citrate, pH 7.0
protocols using SSC
- Dahlquist:DNA Microarray Protocol
- Endy:Northern Blot, 32P End-Labeled Probes
- 20.109(S07): cDNA synthesis and microarray
- 20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray
