SSC

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* 3M NaCl
* 3M NaCl
* 300 mM sodium citrate, pH 7.0  
* 300 mM sodium citrate, pH 7.0  
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== protocols using SSC ==
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* [[Dahlquist:DNA Microarray Protocol]]
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* [[Endy:Northern Blot, 32P End-Labeled Probes]]
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* [[20.109(S07): cDNA synthesis and microarray]]
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* [[20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray]]
== See also ==
== See also ==

Revision as of 08:41, 15 June 2009

SSC buffer is named after its ingredients saline (sodium chloride) and sodium citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.

Contents

1x SSC buffer

  • 150mM NaCl
  • 15mM sodium citrate

20x SSC

  • 800ml dH2O
  • 175.3g NaCl (3M)
  • 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
  • adjust the pH to 7.0 with a few drops of 1M HCl
  • adjust the volume to 1L with dH2O
  • sterilize by autoclaving

using stock solutions

  • 3M NaCl
  • 300 mM sodium citrate, pH 7.0

protocols using SSC

See also

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