SSC

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(20x)
(20x recipe)
Line 4: Line 4:
* 150mM NaCl
* 150mM NaCl
* 15mM sodium citrate
* 15mM sodium citrate
-
 
== 20x SSC ==
== 20x SSC ==
 +
* 800ml dH2O
 +
* 175.3g [http://en.wikipedia.org/wiki/Nacl NaCl] (3M)
 +
* 88.2g [http://en.wikipedia.org/wiki/Trisodium_citrate trisodium citrate] (NaCit; 300mM; e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S4641|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641])
 +
* adjust the pH to 7.0 with a few drops of 1M HCl
 +
* adjust the volume to 1L with dH2O
 +
* sterilize by autoclaving
 +
 +
=== using stock solution ===
* 3M NaCl
* 3M NaCl
* 300 mM sodium citrate, pH 7.0  
* 300 mM sodium citrate, pH 7.0  
[[Category:Material]] and [[Category:Buffers]]
[[Category:Material]] and [[Category:Buffers]]

Revision as of 08:29, 15 June 2009

SSC buffer contains sodium chloride and sodium citrate.

1x SSC buffer

  • 150mM NaCl
  • 15mM sodium citrate

20x SSC

  • 800ml dH2O
  • 175.3g NaCl (3M)
  • 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
  • adjust the pH to 7.0 with a few drops of 1M HCl
  • adjust the volume to 1L with dH2O
  • sterilize by autoclaving

using stock solution

  • 3M NaCl
  • 300 mM sodium citrate, pH 7.0 and
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