SSC
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(Difference between revisions)
(20x) |
(20x recipe) |
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| Line 4: | Line 4: | ||
* 150mM NaCl | * 150mM NaCl | ||
* 15mM sodium citrate | * 15mM sodium citrate | ||
| - | |||
== 20x SSC == | == 20x SSC == | ||
| + | * 800ml dH2O | ||
| + | * 175.3g [http://en.wikipedia.org/wiki/Nacl NaCl] (3M) | ||
| + | * 88.2g [http://en.wikipedia.org/wiki/Trisodium_citrate trisodium citrate] (NaCit; 300mM; e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S4641|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641]) | ||
| + | * adjust the pH to 7.0 with a few drops of 1M HCl | ||
| + | * adjust the volume to 1L with dH2O | ||
| + | * sterilize by autoclaving | ||
| + | |||
| + | === using stock solution === | ||
* 3M NaCl | * 3M NaCl | ||
* 300 mM sodium citrate, pH 7.0 | * 300 mM sodium citrate, pH 7.0 | ||
[[Category:Material]] and [[Category:Buffers]] | [[Category:Material]] and [[Category:Buffers]] | ||
Revision as of 08:29, 15 June 2009
SSC buffer contains sodium chloride and sodium citrate.
1x SSC buffer
- 150mM NaCl
- 15mM sodium citrate
20x SSC
- 800ml dH2O
- 175.3g NaCl (3M)
- 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
- adjust the pH to 7.0 with a few drops of 1M HCl
- adjust the volume to 1L with dH2O
- sterilize by autoclaving
using stock solution
- 3M NaCl
- 300 mM sodium citrate, pH 7.0 and
