http://openwetware.org/index.php?title=SR&feed=atom&action=historySR - Revision history2013-05-24T01:46:51ZRevision history for this page on the wikiMediaWiki 1.13.2http://openwetware.org/index.php?title=SR&diff=434861&oldid=prevDaniel Schlieper: My favourite medium :-)2010-07-22T13:15:26Z<p>My favourite medium :-)</p>
<p><b>New page</b></p><div>== Summary ==<br />
Super Rich Medium (aka SR Medium). This medium is optimized for high-yield protein expression in Escherichia coli.<br />
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== Ingredients ==<br />
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Solution A<br />
# 20 g tryptone<br />
# 20 g yeast extract<br />
# 10 g beef extract<br />
# 11 g glycerol<br />
# 900 mL H<sub>2</sub>O<br />
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Solution B<br />
# 2.32 g KH<sub>2</sub>PO<sub>4</sub><br />
# 16.4 g K<sub>2</sub>HPO<sub>4</sub>*H<sub>2</sub>O<br />
# 100 mL H<sub>2</sub>O<br />
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==Protocol==<br />
# Dissolve ingredients of both solutions. <br />
# Pour into flasks or bottles (Solution A in 2 L flask or greater, Solution B in 250 mL flask or greater).<br />
# Autoclave (liquid cycle)<br />
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==Source==<br />
Feil, I. (1992) PH.D. Thesis, Technische Universtiaet Carolo-Wilhelmina zu Braunschweig, Braunschweig, Germany<br />
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[[Category:Material]] [[Category:Standard Media]]</div>Daniel Schlieper