SOC

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

• SOB
• 20 mM glucose

Protocol

1. Follow directions to make 1 liter of SOB media
2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution

Alternative

1. 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
2. aliquote in 2 ml tubes
3. store at -20°C

Reagent	for 1 L	500 mL	100 mL
2 M glucose	10 ml	5 ml	1 ml

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.