SOC - Revision history

Etienne Robillard: fixed heading

Etienne Robillard — Fri, 25 Jan 2013 22:33:29 GMT

fixed heading

←Older revision		Revision as of 22:33, 25 January 2013
Line 11:		Line 11:


-	===Alternative==	+	==Alternative==
	#2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube		#2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
	#aliquote in 2 ml tubes		#aliquote in 2 ml tubes

Christopher C Vanlang: /* Protocol */

Christopher C Vanlang — Fri, 30 Dec 2011 03:09:07 GMT

Protocol

←Older revision		Revision as of 03:09, 30 December 2011
Line 11:		Line 11:


-	*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube	+	===Alternative==
-		+	#2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
		+	#aliquote in 2 ml tubes
		+	#store at -20°C
	{\| border="1" cellpadding="5" cellspacing="0"		{\| border="1" cellpadding="5" cellspacing="0"
	\|+		\|+
Line 25:		Line 27:
	\| 1 ml		\| 1 ml
	\|}		\|}
-
-	*aliquote in 2 ml tubes
-	*store at -20°C

	==Source==		==Source==

Christopher C Vanlang: /* Protocol */

Christopher C Vanlang — Fri, 30 Dec 2011 03:08:07 GMT

Protocol

←Older revision		Revision as of 03:08, 30 December 2011
Line 9:		Line 9:
	#Follow directions to make 1 liter of [[SOB]] media		#Follow directions to make 1 liter of [[SOB]] media
	#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution		#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
		+

	*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube		*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube

-	{\| border="1" cellpadding="5" cellspacing="0~~" align="left~~"	+	{\| border="1" cellpadding="5" cellspacing="0"
	\|+		\|+
	! Reagent		! Reagent
Line 24:		Line 25:
	\| 1 ml		\| 1 ml
	\|}		\|}
-

	*aliquote in 2 ml tubes		*aliquote in 2 ml tubes

Christopher C Vanlang: /* Protocol */

Christopher C Vanlang — Fri, 30 Dec 2011 03:06:28 GMT

Protocol

←Older revision		Revision as of 03:06, 30 December 2011
Line 24:		Line 24:
	\| 1 ml		\| 1 ml
	\|}		\|}
		+
		+
	*aliquote in 2 ml tubes		*aliquote in 2 ml tubes
	*store at -20°C		*store at -20°C

Christopher C Vanlang: /* Protocol */

Christopher C Vanlang — Fri, 30 Dec 2011 03:05:58 GMT

Protocol

←Older revision		Revision as of 03:05, 30 December 2011
Line 9:		Line 9:
	#Follow directions to make 1 liter of [[SOB]] media		#Follow directions to make 1 liter of [[SOB]] media
	#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution		#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
		+
		+	*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
		+
		+	{\| border="1" cellpadding="5" cellspacing="0" align="left"
		+	\|+
		+	! Reagent
		+	! for 1 L
		+	! 500 mL
		+	! 100 mL
		+	\|-=
		+	\| 2 M glucose
		+	\|10 ml
		+	\| 5 ml
		+	\| 1 ml
		+	\|}
		+	*aliquote in 2 ml tubes
		+	*store at -20°C

	==Source==		==Source==

Torsten Waldminghaus: added tags

Torsten Waldminghaus — Tue, 11 Mar 2008 21:12:09 GMT

added tags

←Older revision		Revision as of 21:12, 11 March 2008
Line 17:		Line 17:
	==Trivia==		==Trivia==
	The "C" in SOC supposedly stands for catabolite repression due to the added glucose.		The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
		+
		+	[[Category:Material]] [[Category:Standard Media]]

Austin J. Che at 17:45, 15 April 2006

Austin J. Che — Sat, 15 Apr 2006 17:45:22 GMT

←Older revision		Revision as of 17:45, 15 April 2006
Line 1:		Line 1:
	== Summary ==		== Summary ==
-	SOC Medium. Used in ~~bacterial transformations~~	+	SOC Medium. Used in [[Bacterial Transformation]].
		+
	== Ingredients ==		== Ingredients ==
-	*~~0.5% (w/v) yeast extract~~	+	*[[SOB]]
-	*2% (w/v) tryptone	+
-	*10 mM NaCl	+
-	*2.5 mM KCl	+
-	*10 mM MgCl<sub>2</sub>	+
-	*20 mM MgSO<sub>4</sub>	+
	*20 mM glucose		*20 mM glucose
		+
	==Protocol==		==Protocol==
-	#SOB ~~(SOC - glucose) is available at the kitchen~~	+	#Follow directions to make 1 liter of [[SOB]] media
-	#~~Add 1/100 volume 40~~% glucose ~~(also available from the kitchen)~~	+	#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution

	==Source==		==Source==
Line 17:		Line 14:

	F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36		F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
		+
		+	==Trivia==
		+	The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

Jgritton at 20:02, 6 June 2005

Jgritton — Mon, 06 Jun 2005 20:02:54 GMT

New page

== Summary ==
SOC Medium. Used in bacterial transformations
== Ingredients ==
*0.5% (w/v) yeast extract
*2% (w/v) tryptone
*10 mM NaCl
*2.5 mM KCl
*10 mM MgCl<sub>2</sub>
*20 mM MgSO<sub>4</sub>
*20 mM glucose
==Protocol==
#SOB (SOC - glucose) is available at the kitchen
#Add 1/100 volume 40% glucose (also available from the kitchen)

==Source==
Adapted From:

F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36