SOC: Difference between revisions
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== Summary == | == Summary == | ||
SOC Medium. Used in | SOC Medium. Used in [[Bacterial Transformation]]. | ||
== Ingredients == | == Ingredients == | ||
* | *[[SOB]] | ||
*20 mM glucose | *20 mM glucose | ||
==Protocol== | ==Protocol== | ||
#SOB | #Follow directions to make 1 liter of [[SOB]] media | ||
# | #After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | ||
==Source== | ==Source== | ||
| Line 17: | Line 14: | ||
F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36 | F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36 | ||
==Trivia== | |||
The "C" in SOC supposedly stands for catabolite repression due to the added glucose. | |||
Revision as of 10:45, 15 April 2006
Summary
SOC Medium. Used in Bacterial Transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Follow directions to make 1 liter of SOB media
- After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
