SOC: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(added tags) |
||
| Line 17: | Line 17: | ||
==Trivia== | ==Trivia== | ||
The "C" in SOC supposedly stands for catabolite repression due to the added glucose. | The "C" in SOC supposedly stands for catabolite repression due to the added glucose. | ||
[[Category:Material]] [[Category:Standard Media]] | |||
Revision as of 14:12, 11 March 2008
Summary
SOC Medium. Used in Bacterial Transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Follow directions to make 1 liter of SOB media
- After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
