SOC: Difference between revisions
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#Follow directions to make 1 liter of [[SOB]] media | #Follow directions to make 1 liter of [[SOB]] media | ||
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | #After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | ||
*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube | *2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube | ||
{| border="1" cellpadding="5" cellspacing="0 | {| border="1" cellpadding="5" cellspacing="0" | ||
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! Reagent | ! Reagent | ||
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| 1 ml | | 1 ml | ||
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*aliquote in 2 ml tubes | *aliquote in 2 ml tubes | ||
Revision as of 20:08, 29 December 2011
Summary
SOC Medium. Used in Bacterial Transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Follow directions to make 1 liter of SOB media
- After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
- 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
| Reagent | for 1 L | 500 mL | 100 mL |
|---|---|---|---|
| 2 M glucose | 10 ml | 5 ml | 1 ml |
- aliquote in 2 ml tubes
- store at -20°C
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
