SOC

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
== Summary ==
== Summary ==
-
SOC Medium.  Used in bacterial transformations
+
SOC Medium.  Used in [[Bacterial Transformation]].
 +
 
== Ingredients ==
== Ingredients ==
-
*0.5% (w/v) yeast extract
+
*[[SOB]]
-
*2% (w/v) tryptone
+
-
*10 mM NaCl
+
-
*2.5 mM KCl
+
-
*10 mM MgCl<sub>2</sub>
+
-
*20 mM MgSO<sub>4</sub>
+
*20 mM glucose
*20 mM glucose
 +
==Protocol==
==Protocol==
-
#SOB (SOC - glucose) is available at the kitchen
+
#Follow directions to make 1 liter of [[SOB]] media
-
#Add 1/100 volume 40% glucose (also available from the kitchen)
+
#After cooling medium to less than 50&deg;C, add 20 ml filter sterilized 20% glucose solution
==Source==
==Source==
Line 17: Line 14:
F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
 +
 +
==Trivia==
 +
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

Revision as of 13:45, 15 April 2006

Contents

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

Personal tools