SOC

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==Trivia==
==Trivia==
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
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[[Category:Material]]  [[Category:Standard Media]]

Revision as of 17:12, 11 March 2008

Contents

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

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