SOB - Revision history

Christopher C Vanlang at 03:12, 30 December 2011

Christopher C Vanlang — Fri, 30 Dec 2011 03:12:15 GMT

←Older revision		Revision as of 03:12, 30 December 2011
Line 31:		Line 31:
	* 10 mM MgCl<sub>2</sub>		* 10 mM MgCl<sub>2</sub>
	* 10 mM MgSO<sub>4</sub>		* 10 mM MgSO<sub>4</sub>
		+
		+	==Extended instructions==
		+
		+	*1 M MgCl2 stock: dissolve 20.33 g MgCl2 6H2O in 100 ml ddH2O (XXX), autoclave on liquid cycle @ XXX°C for 20 min (can be done at the same time as SOC pre-mix below)
		+	*250 mM KCl stock: dissolve 1.86 KCl in 100 ml ddH2O (XXX)
		+
		+	*combine:
		+	{\| border="1" cellpadding="5" cellspacing="0"
		+	\|+
		+	! Reagent
		+	! for 1 L
		+	! 500 mL
		+	! 100 mL
		+	\|-=
		+	\| tryptone
		+	\| 20 g
		+	\| 10 g
		+	\| 2g
		+	\|-
		+	\| yeast
		+	\| 5g
		+	\| 2.5 g
		+	\|0.5 g
		+	\|-
		+	\| NaCl
		+	\|0.5 g
		+	\| 0.25 g
		+	\|0.05 g
		+	\|-
		+	\| 250 nM KCl
		+	\|10 mL
		+	\|5mL
		+	\|1 mL
		+	\|-
		+	\| ddH2O
		+	\| 980 ml
		+	\| 490 ml
		+	\| 98 ml
		+	\|}
		+	*adjust pH to 7.0 w/ NaOH
		+	*bring to volume:
		+
		+	{\| border="1" cellpadding="5" cellspacing="0"
		+	\|+
		+	! Reagent
		+	! for 1 L
		+	! 500 mL
		+	! 100 mL
		+
		+	\|}
		+	*autoclave on liquid cycle @ XXX°C for 20 min
		+
		+	*add autoclaved 1 M MgCl2
		+
		+	{\| border="1" cellpadding="5" cellspacing="0"
		+	\|+
		+	! Reagent
		+	! for 1 L
		+	! 500 mL
		+	! 100 mL
		+	\|-=
		+	\| 1 M MgCl2
		+	\| 10 ml
		+	\| 5 ml
		+	\| 1 ml
		+	\|}

	==Source==		==Source==

Torsten Waldminghaus: added tags

Torsten Waldminghaus — Tue, 11 Mar 2008 21:11:44 GMT

added tags

←Older revision		Revision as of 21:11, 11 March 2008
Line 39:		Line 39:
	==Trivia==		==Trivia==
	SOB supposedly stands for super optimal broth.		SOB supposedly stands for super optimal broth.
		+
		+	[[Category:Material]] [[Category:Standard Media]]

Tk: /* 15/10 medium */

Tk — Tue, 09 May 2006 21:04:02 GMT

15/10 medium

←Older revision		Revision as of 21:04, 9 May 2006
Line 23:		Line 23:

	==15/10 medium==		==15/10 medium==
-	Growth of competent TOP10 cells in the ~~Jessee~~ patent is performed in 15/10 broth, which is similar to SOB:	+	Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:

	* 1.5% yeast extract		* 1.5% yeast extract
Line 31:		Line 31:
	* 10 mM MgCl<sub>2</sub>		* 10 mM MgCl<sub>2</sub>
	* 10 mM MgSO<sub>4</sub>		* 10 mM MgSO<sub>4</sub>
-

	==Source==		==Source==

Tk at 15:35, 9 May 2006

Tk — Tue, 09 May 2006 15:35:51 GMT

←Older revision		Revision as of 15:35, 9 May 2006
Line 21:		Line 21:

	Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.		Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
		+
		+	==15/10 medium==
		+	Growth of competent TOP10 cells in the Jessee patent is performed in 15/10 broth, which is similar to SOB:
		+
		+	* 1.5% yeast extract
		+	* 1% Bacto-Tryptone
		+	* 10mM NaCl
		+	* 2mM KCl
		+	* 10 mM MgCl<sub>2</sub>
		+	* 10 mM MgSO<sub>4</sub>
		+

	==Source==		==Source==

Tk at 15:28, 9 May 2006

Tk — Tue, 09 May 2006 15:28:48 GMT

←Older revision		Revision as of 15:28, 9 May 2006
Line 16:		Line 16:
	*2.4 g MgSO<sub>4</sub>		*2.4 g MgSO<sub>4</sub>

-	''Note:'' Some formulations of SOB ~~also include~~ 10 mM MgCl<sub>2</sub>.	+	''Note:'' Some formulations of SOB use 10 mM MgCl<sub>2</sub> and 10 mM MgSO<sub>4</sub> instead of 20 mM MgSO<sub>4</sub>.

	SOB medium is also available dry premixed from Difco, 0443-17.		SOB medium is also available dry premixed from Difco, 0443-17.

Tk: /* Ingredients */

Tk — Tue, 25 Apr 2006 19:56:21 GMT

Ingredients

←Older revision		Revision as of 19:56, 25 April 2006
Line 17:		Line 17:

	''Note:'' Some formulations of SOB also include 10 mM MgCl<sub>2</sub>.		''Note:'' Some formulations of SOB also include 10 mM MgCl<sub>2</sub>.
		+
		+	SOB medium is also available dry premixed from Difco, 0443-17.
		+
		+	Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.

	==Source==		==Source==

Austin J. Che at 17:45, 15 April 2006

Austin J. Che — Sat, 15 Apr 2006 17:45:13 GMT

←Older revision		Revision as of 17:45, 15 April 2006
Line 7:		Line 7:
	*10 mM NaCl		*10 mM NaCl
	*2.5 mM KCl		*2.5 mM KCl
-	*10 mM MgCl<sub>2</sub>
	*20 mM MgSO<sub>4</sub>		*20 mM MgSO<sub>4</sub>
		+
		+	Per liter:
		+	*5 g yeast extract
		+	*20 g tryptone
		+	*0.584 g NaCl
		+	*0.186 g KCl
		+	*2.4 g MgSO<sub>4</sub>
		+
		+	''Note:'' Some formulations of SOB also include 10 mM MgCl<sub>2</sub>.

	==Source==		==Source==
Line 14:		Line 22:

	F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36		F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
		+
		+	==Trivia==
		+	SOB supposedly stands for super optimal broth.

Tk at 01:35, 12 April 2006

Tk — Wed, 12 Apr 2006 01:35:32 GMT

New page

== Summary ==
SOB Medium. Used in growing bacteria for preparing chemically competent cells

== Ingredients ==
*0.5% (w/v) yeast extract
*2% (w/v) tryptone
*10 mM NaCl
*2.5 mM KCl
*10 mM MgCl<sub>2</sub>
*20 mM MgSO<sub>4</sub>

==Source==
Adapted From:

F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36