SOB

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(15/10 medium)
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==15/10 medium==
==15/10 medium==
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Growth of competent TOP10 cells in the Jessee patent is performed in 15/10 broth, which is similar to SOB:
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Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:
* 1.5% yeast extract
* 1.5% yeast extract
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* 10 mM MgCl<sub>2</sub>
* 10 mM MgCl<sub>2</sub>
* 10 mM MgSO<sub>4</sub>
* 10 mM MgSO<sub>4</sub>
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==Source==
==Source==

Revision as of 16:04, 9 May 2006

Contents

Summary

SOB Medium. Used in growing bacteria for preparing chemically competent cells

Ingredients

  • 0.5% (w/v) yeast extract
  • 2% (w/v) tryptone
  • 10 mM NaCl
  • 2.5 mM KCl
  • 20 mM MgSO4

Per liter:

  • 5 g yeast extract
  • 20 g tryptone
  • 0.584 g NaCl
  • 0.186 g KCl
  • 2.4 g MgSO4

Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.

SOB medium is also available dry premixed from Difco, 0443-17.

Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.

15/10 medium

Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:

  • 1.5% yeast extract
  • 1% Bacto-Tryptone
  • 10mM NaCl
  • 2mM KCl
  • 10 mM MgCl2
  • 10 mM MgSO4

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

SOB supposedly stands for super optimal broth.

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