SOB: Difference between revisions
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Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | ||
==15/10 medium== | |||
Growth of competent TOP10 cells in the Jessee patent is performed in 15/10 broth, which is similar to SOB: | |||
* 1.5% yeast extract | |||
* 1% Bacto-Tryptone | |||
* 10mM NaCl | |||
* 2mM KCl | |||
* 10 mM MgCl<sub>2</sub> | |||
* 10 mM MgSO<sub>4</sub> | |||
==Source== | ==Source== |
Revision as of 08:35, 9 May 2006
Summary
SOB Medium. Used in growing bacteria for preparing chemically competent cells
Ingredients
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
SOB medium is also available dry premixed from Difco, 0443-17.
Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
15/10 medium
Growth of competent TOP10 cells in the Jessee patent is performed in 15/10 broth, which is similar to SOB:
- 1.5% yeast extract
- 1% Bacto-Tryptone
- 10mM NaCl
- 2mM KCl
- 10 mM MgCl2
- 10 mM MgSO4
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
SOB supposedly stands for super optimal broth.