SOB

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Adjust to pH 7.5 prior to use.  This requires approximately 25 ml of 1M NaOH per liter.
Adjust to pH 7.5 prior to use.  This requires approximately 25 ml of 1M NaOH per liter.
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==15/10 medium==
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Growth of competent TOP10 cells in the Jessee patent is performed in 15/10 broth, which is similar to SOB:
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* 1.5% yeast extract
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* 1% Bacto-Tryptone
 +
* 10mM NaCl
 +
* 2mM KCl
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* 10 mM MgCl<sub>2</sub>
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* 10 mM MgSO<sub>4</sub>
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==Source==
==Source==

Revision as of 10:35, 9 May 2006

Contents

Summary

SOB Medium. Used in growing bacteria for preparing chemically competent cells

Ingredients

  • 0.5% (w/v) yeast extract
  • 2% (w/v) tryptone
  • 10 mM NaCl
  • 2.5 mM KCl
  • 20 mM MgSO4

Per liter:

  • 5 g yeast extract
  • 20 g tryptone
  • 0.584 g NaCl
  • 0.186 g KCl
  • 2.4 g MgSO4

Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.

SOB medium is also available dry premixed from Difco, 0443-17.

Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.

15/10 medium

Growth of competent TOP10 cells in the Jessee patent is performed in 15/10 broth, which is similar to SOB:

  • 1.5% yeast extract
  • 1% Bacto-Tryptone
  • 10mM NaCl
  • 2mM KCl
  • 10 mM MgCl2
  • 10 mM MgSO4


Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

SOB supposedly stands for super optimal broth.

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