SOB

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Line 7: Line 7:
*10 mM NaCl
*10 mM NaCl
*2.5 mM KCl
*2.5 mM KCl
-
*10 mM MgCl<sub>2</sub>
 
*20 mM MgSO<sub>4</sub>
*20 mM MgSO<sub>4</sub>
 +
 +
Per liter:
 +
*5 g yeast extract
 +
*20 g tryptone
 +
*0.584 g NaCl
 +
*0.186 g KCl
 +
*2.4 g MgSO<sub>4</sub>
 +
 +
''Note:'' Some formulations of SOB also include 10 mM MgCl<sub>2</sub>.
==Source==
==Source==
Line 14: Line 22:
F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
 +
 +
==Trivia==
 +
SOB supposedly stands for super optimal broth.

Revision as of 12:45, 15 April 2006

Contents

Summary

SOB Medium. Used in growing bacteria for preparing chemically competent cells

Ingredients

  • 0.5% (w/v) yeast extract
  • 2% (w/v) tryptone
  • 10 mM NaCl
  • 2.5 mM KCl
  • 20 mM MgSO4

Per liter:

  • 5 g yeast extract
  • 20 g tryptone
  • 0.584 g NaCl
  • 0.186 g KCl
  • 2.4 g MgSO4

Note: Some formulations of SOB also include 10 mM MgCl2.

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

SOB supposedly stands for super optimal broth.

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