SEED/2012/Schedule

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Overview pages

• [[../Overview/]] of lab sequence & procedures
• [[../Design Project/]] description

Course schedule

Class	Date	Lecture	Homework (due next week)	Lab
[[../Day 1/]]	Feb. 4	Introductions Molecular Biology Review Units Review Course description & requirements Diagnostic test	Homework #1: read Adventures in Synthbio comic Think of >=3 ideas for what you want to engineer biology to do Pre-lab #1 Post-lab #1	Lab Day #1: doc pdf Lab safety Concentrations & dilutions Using a micropipet Serial dilutions & spectrophotometer
[[../Day 2/]]	Feb. 11	Goals of SynthBio What makes a good standardized part Why biology is hard to engineer Parts/devices/systems hierarchy and black-boxing Working example: Bacterial Photography System Discuss Adventures in Synthbio and project ideas	Homework #2: doc pdf Post-Lab #2 Pre-Lab #3	Lab Day #2: doc pdf Overview of cloning process Minipreps Pre-Lab #2
No Class	Feb. 19	Presidents' Day
[[../Day 3/]]	Feb. 25	Polymerase chain reaction	Homework #3: doc pdf Central Dogma review reading: Cartoon Guide to Genetics Post-Lab #3 Pre-Lab #4	Lab Day #3: doc pdf Polymerase Chain Reaction (PCR)
[[../Day 4/]]	Mar. 3	Restriction digest	Homework #4: Three Ideas Report doc pdf (rough draft due Day 5, final draft due Day 6) Post-Lab #4 Pre-Lab #5	Lab Day #4: doc pdf Restriction Digest
[[../Day 5/]]	Mar. 10	Gel Electrophoresis & Purification	Homework #5: Finish Three Ideas Report Post-Lab #5 Pre-Lab #6	Lab Day #5: doc pdf Gel Electrophoresis
[[../Day 6/]]	Mar. 17	Ligation & transformation	Homework #6: to be posted Post-Lab #6 Pre-Lab #7	Lab Day #6: doc pdf Note: posted lab packet does not currently include protocol, but includes all information necessary to do homework Ligation (morning) Transformation (afternoon)
[[../Day 7/]]	Mar. 24	Sequencing to Verify Plasmids Preparing Freezer Stocks	Homework #7: to be posted Post-Lab #7 Pre-Lab #8	Lab Day #7: to be posted Miniprep for sequencing Prepare sequencing reaction to verify transformed strains Making glycerol freezer stocks of transformed strains
No SEED	Mar. 31
[[../Day 8/]]	Apr. 7	Analyzing sequencing data Qualitative vs. quantitative measurements	Homework #8: to be posted Post-Lab #8 Pre-Lab #9	Lab Day #8: to be posted Analyzing sequencing results Measuring induction of fluorescent proteins
[[../Day 9/]]	Apr. 14	Projects!	Homework #9: to be posted Post-lab #9	Lab Day #9: to be posted Measuring LacZ expression with various promoter/RBS combos
No SEED	Apr. 21
[[../Day 10/]]	April 28	Designing a poster Presenting a poster	Prepare to present projects!	Project work time
	May 4	Final Presentations	Kirsch Auditorium (room 32-123), Stata Center 6:30pm-8pm