SEED/2010/Schedule
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Current revision (14:58, 17 February 2010) (view source) |
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| [[../Day 1/]] | | [[../Day 1/]] | ||
| - | | Feb. | + | | Feb. 6 |
| safety training, [[Media:7.02-training-lab.pdf|training lab]] | | safety training, [[Media:7.02-training-lab.pdf|training lab]] | ||
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| [[../Day 2/]] | | [[../Day 2/]] | ||
| - | | Feb. | + | | Feb. 20 |
| SynBio Overview, sterile technique, colony streaking, antibiotic resistance | | SynBio Overview, sterile technique, colony streaking, antibiotic resistance | ||
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| [[../Day 3/]] | | [[../Day 3/]] | ||
| - | | Feb. | + | | Feb. 27 |
| miniprep, digest | | miniprep, digest | ||
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| [[../Day 4/]] | | [[../Day 4/]] | ||
| - | | Mar. | + | | Mar. 6 |
| PCR and gel | | PCR and gel | ||
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| [[../Day 5/]] | | [[../Day 5/]] | ||
| - | | Mar. | + | | Mar. 13 |
| gel purification: [[Media:SEED_Gel_pur-2009.pdf|pdf]] [[Media:SEED_Gel_pur-2009.docx|docx]] | | gel purification: [[Media:SEED_Gel_pur-2009.pdf|pdf]] [[Media:SEED_Gel_pur-2009.docx|docx]] | ||
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| [[../Day 6/]] | | [[../Day 6/]] | ||
| - | | Mar. | + | | Mar. 20 |
| ligation, transformation | | ligation, transformation | ||
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| [[../Day 7/]] | | [[../Day 7/]] | ||
| - | | Mar. | + | | Mar. 27 |
| prep for sequencing, glycerol | | prep for sequencing, glycerol | ||
final project notes: | final project notes: | ||
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| [[../Day 8/]] | | [[../Day 8/]] | ||
| - | | Apr. | + | | Apr. 3 |
| β-gal assays, growth curves, viable cell titer, analyze sequencing | | β-gal assays, growth curves, viable cell titer, analyze sequencing | ||
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| [[../Day 9/]] | | [[../Day 9/]] | ||
| - | | Apr. | + | | Apr. 17 |
| device characterization and composability | | device characterization and composability | ||
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| [[../Day 10/]] | | [[../Day 10/]] | ||
| - | | May | + | | May 1 |
| data analysis, finish projects | | data analysis, finish projects | ||
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Current revision
Overview of the main lab project
| Class | Date | Agenda | Homework | Lab Protocol |
|---|---|---|---|---|
| Day 1 | Feb. 6 | safety training, training lab | Lab Day #1: Serial Dilutions pdf doc | |
| Day 2 | Feb. 20 | SynBio Overview, sterile technique, colony streaking, antibiotic resistance |
| Lab Day #2: Plate Streaking pdf doc |
| Day 3 | Feb. 27 | miniprep, digest | ||
| Day 4 | Mar. 6 | PCR and gel | ||
| Day 5 | Mar. 13 | gel purification: pdf docx | Lab Day #5: Gel Purification pdf doc | |
| Day 6 | Mar. 20 | ligation, transformation |
| Lab Day #6: Ligation pdf doc |
| Day 7 | Mar. 27 | prep for sequencing, glycerol
final project notes:
| Lab Day #7: Transformation pdf doc | |
| Day 8 | Apr. 3 | β-gal assays, growth curves, viable cell titer, analyze sequencing |
| Lab Day #8: Beta-Galactosidase: Part I pdf doc |
| Day 9 | Apr. 17 | device characterization and composability |
| Lab Day #9: Beta-Galactosidase: Part II pdf doc |
| Day 10 | May 1 | data analysis, finish projects |
| Final Lab: pdf |
