SEED/2010/Schedule
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Revision as of 13:48, 2 February 2010
Overview of the main lab project
| Class | Date | Agenda | Homework | Lab Protocol |
|---|---|---|---|---|
| Day 1 | Feb. 7 | safety training, training lab | Lab Day #1: Serial Dilutions pdf doc | |
| Day 2 | Feb. 21 | SynBio Overview, sterile technique, colony streaking, antibiotic resistance |
| Lab Day #2: Plate Streaking pdf doc |
| Day 3 | Feb. 28 | miniprep, digest | ||
| Day 4 | Mar. 7 | PCR and gel | ||
| Day 5 | Mar. 14 | gel purification: pdf docx | Lab Day #5: Gel Purification pdf doc | |
| Day 6 | Mar. 21 | ligation, transformation |
| Lab Day #6: Ligation pdf doc |
| Day 7 | Mar. 28 | prep for sequencing, glycerol
final project notes:
| Lab Day #7: Transformation pdf doc | |
| Day 8 | Apr. 4 | β-gal assays, growth curves, viable cell titer, analyze sequencing |
| Lab Day #8: Beta-Galactosidase: Part I pdf doc |
| Day 9 | Apr. 18 | device characterization and composability |
| Lab Day #9: Beta-Galactosidase: Part II pdf doc |
| Day 10 | May 2 | data analysis, finish projects |
| Final Lab: pdf |
