SEED/2009/Schedule: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 23: | Line 23: | ||
|- | |- | ||
| [[../Day 1/]] | | [[../Day 1/]] | ||
| Feb. | | Feb. 6 | ||
| safety training, [[Media:7.02-training-lab.pdf|training lab]] | | safety training, [[Media:7.02-training-lab.pdf|training lab]] | ||
| | | | ||
| Line 36: | Line 36: | ||
|- | |- | ||
| [[../Day 2/]] | | [[../Day 2/]] | ||
| Feb. | | Feb. 20 | ||
| SynBio Overview, sterile technique, colony streaking, antibiotic resistance | | SynBio Overview, sterile technique, colony streaking, antibiotic resistance | ||
| | | | ||
| Line 47: | Line 47: | ||
|- | |- | ||
| [[../Day 3/]] | | [[../Day 3/]] | ||
| Feb. | | Feb. 27 | ||
| miniprep, digest | | miniprep, digest | ||
| | | | ||
| Line 57: | Line 57: | ||
|- | |- | ||
| [[../Day 4/]] | | [[../Day 4/]] | ||
| Mar. | | Mar. 6 | ||
| PCR and gel | | PCR and gel | ||
| | | | ||
| Line 66: | Line 66: | ||
|- | |- | ||
| [[../Day 5/]] | | [[../Day 5/]] | ||
| Mar. | | Mar. 13 | ||
| gel purification: [[Media:SEED_Gel_pur-2009.pdf|pdf]] [[Media:SEED_Gel_pur-2009.docx|docx]] | | gel purification: [[Media:SEED_Gel_pur-2009.pdf|pdf]] [[Media:SEED_Gel_pur-2009.docx|docx]] | ||
| | | | ||
| Line 74: | Line 74: | ||
|- | |- | ||
| [[../Day 6/]] | | [[../Day 6/]] | ||
| Mar. | | Mar. 20 | ||
| ligation, transformation | | ligation, transformation | ||
| | | | ||
| Line 82: | Line 82: | ||
|- | |- | ||
| [[../Day 7/]] | | [[../Day 7/]] | ||
| | | Apr. 3 | ||
| prep for sequencing, glycerol | | prep for sequencing, glycerol | ||
final project notes: | final project notes: | ||
| Line 93: | Line 93: | ||
|- | |- | ||
| [[../Day 8/]] | | [[../Day 8/]] | ||
| Apr. | | Apr. 10 | ||
| β-gal assays, growth curves, viable cell titer, analyze sequencing | | β-gal assays, growth curves, viable cell titer, analyze sequencing | ||
| | | | ||
| Line 102: | Line 102: | ||
|- | |- | ||
| [[../Day 9/]] | | [[../Day 9/]] | ||
| Apr. | | Apr. 24 | ||
| device characterization and composability | | device characterization and composability | ||
| | | | ||
| Line 109: | Line 109: | ||
|- | |- | ||
| [[../Day 10/]] | | [[../Day 10/]] | ||
| May | | May 1 | ||
| data analysis, finish projects | | data analysis, finish projects | ||
| | | | ||
Latest revision as of 10:55, 2 February 2010
http://web.mit.edu/seed/images/seed.gif
[[../Overview/]] of the main lab project
| Class | Date | Agenda | Homework | Lab Protocol |
|---|---|---|---|---|
| [[../Day 1/]] | Feb. 6 | safety training, training lab | Lab Day #1: Serial Dilutions pdf doc | |
| [[../Day 2/]] | Feb. 20 | SynBio Overview, sterile technique, colony streaking, antibiotic resistance |
|
Lab Day #2: Plate Streaking pdf doc |
| [[../Day 3/]] | Feb. 27 | miniprep, digest | ||
| [[../Day 4/]] | Mar. 6 | PCR and gel | ||
| [[../Day 5/]] | Mar. 13 | gel purification: pdf docx | Lab Day #5: Gel Purification pdf doc | |
| [[../Day 6/]] | Mar. 20 | ligation, transformation |
|
Lab Day #6: Ligation pdf doc |
| [[../Day 7/]] | Apr. 3 | prep for sequencing, glycerol
final project notes:
|
Lab Day #7: Transformation pdf doc | |
| [[../Day 8/]] | Apr. 10 | β-gal assays, growth curves, viable cell titer, analyze sequencing |
|
Lab Day #8: Beta-Galactosidase: Part I pdf doc |
| [[../Day 9/]] | Apr. 24 | device characterization and composability |
|
Lab Day #9: Beta-Galactosidase: Part II pdf doc |
| [[../Day 10/]] | May 1 | data analysis, finish projects |
|
Final Lab: pdf |
