SEED/2009/Schedule: Difference between revisions
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** Data Analysis | ** Data Analysis | ||
** Pre-Lab | ** Pre-Lab | ||
| Lab Day #1: pdf [[Media:SEED_pipette.doc| | | Lab Day #1: pdf [[Media:SEED_pipette.doc|Serial Dilutions]] | ||
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| [[../Day 2/]] | | [[../Day 2/]] | ||
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* Pre-Lab: [[Media:PreLab2-2009.doc|doc]] [[Media:PreLab2-2009.pdf|pdf]] | * Pre-Lab: [[Media:PreLab2-2009.doc|doc]] [[Media:PreLab2-2009.pdf|pdf]] | ||
* Quiz 1: [[Media:SEED Quiz1 2009.doc|doc]] [[Media:SEED Quiz1 2009.pdf|pdf]] | * Quiz 1: [[Media:SEED Quiz1 2009.doc|doc]] [[Media:SEED Quiz1 2009.pdf|pdf]] | ||
| Lab Day #2: pdf [[Media:SEED_plate_streaking.doc|doc]] | | Lab Day #2: Plate Streaking [[Media:SEED_plate_streaking.pdf|pdf]] [[Media:SEED_plate_streaking.doc|doc]] | ||
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| [[../Day 3/]] | | [[../Day 3/]] | ||
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* Homework #3: [[Media:SEED_HW3-2009.doc|doc]] [[Media:SEED_HW3-2009.pdf|pdf]] | * Homework #3: [[Media:SEED_HW3-2009.doc|doc]] [[Media:SEED_HW3-2009.pdf|pdf]] | ||
* Pre-Lab #3: [[Media:SEED_PreLab3-2009.doc|doc]] [[Media:SEED_PreLab3-2009.pdf|pdf]] | * Pre-Lab #3: [[Media:SEED_PreLab3-2009.doc|doc]] [[Media:SEED_PreLab3-2009.pdf|pdf]] | ||
| Lab Day #3: pdf [[Media:SEED_mini_prep.doc|doc]][[Media:SEED_digest.doc|doc]] | | | ||
* Lab Day #3: Plasmid Mini-Prep [[Media:SEED_mini_prep.pdf|pdf]] [[Media:SEED_mini_prep.doc|doc]] | |||
* Restriction Digest [[Media:SEED_digest.pdf|pdf]] [[Media:SEED_digest.doc|doc]] | |||
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| [[../Day 4/]] | | [[../Day 4/]] | ||
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* Homework #4: [[Media:SEED_HW4-2009.doc|doc]] [[Media:SEED_HW4-2009.pdf|pdf]] | * Homework #4: [[Media:SEED_HW4-2009.doc|doc]] [[Media:SEED_HW4-2009.pdf|pdf]] | ||
| Lab Day #4: pdf [[Media:SEED_PCR.doc|doc]] [[Media:SEED_gel_electrophoresis.doc|doc]] | | | ||
* Lab Day #4: PCR [[Media:SEED_PCR.pdf|pdf]] [[Media:SEED_PCR.doc|doc]] | |||
* Gel Electrophoresis [[Media:SEED_gel_electrophoresis.pdf|pdf]] [[Media:SEED_gel_electrophoresis.doc|doc]] | |||
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| [[../Day 5/]] | | [[../Day 5/]] | ||
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* Homework #5: [[Media:SEED_HW5-2009.doc|docx]] [[Media:SEED_HW5-2009.pdf|pdf]] | * Homework #5: [[Media:SEED_HW5-2009.doc|docx]] [[Media:SEED_HW5-2009.pdf|pdf]] | ||
* Quiz 2: [[Media:SEED Quiz2 2009.doc|doc]] [[Media:SEED Quiz2 2009.pdf|pdf]] | * Quiz 2: [[Media:SEED Quiz2 2009.doc|doc]] [[Media:SEED Quiz2 2009.pdf|pdf]] | ||
| Lab Day #5: pdf [[Media:SEED_gel_purification.doc|doc]] | | Lab Day #5: Gel Purification [[Media:SEED_gel_purification.pdf|pdf]] [[Media:SEED_gel_purification.doc|doc]] | ||
|- | |- | ||
| [[../Day 6/]] | | [[../Day 6/]] | ||
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* Pre-Lab #6: [http://www.zerobio.com/videos/sanger.html sequencing] | * Pre-Lab #6: [http://www.zerobio.com/videos/sanger.html sequencing] | ||
* Quiz 3: [[Media:SEED Quiz3 2009.doc|doc]] [[Media:SEED Quiz3 2009.pdf|pdf]] | * Quiz 3: [[Media:SEED Quiz3 2009.doc|doc]] [[Media:SEED Quiz3 2009.pdf|pdf]] | ||
| Lab Day #6: pdf [[Media:SEED_ligation.doc|doc]] | | Lab Day #6: Ligation [[Media:SEED_ligation.pdf|pdf]] [[Media:SEED_ligation.doc|doc]] | ||
|- | |- | ||
| [[../Day 7/]] | | [[../Day 7/]] | ||
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* Pre-Lab #5: [[Media:SEED_PL5-2009.doc|doc]] [[Media:SEED_PL5-2009.pdf|pdf]] | * Pre-Lab #5: [[Media:SEED_PL5-2009.doc|doc]] [[Media:SEED_PL5-2009.pdf|pdf]] | ||
* Quiz 4: [[Media:SEED Quiz4 2009.doc|doc]] [[Media:SEED Quiz4 2009.pdf|pdf]] | * Quiz 4: [[Media:SEED Quiz4 2009.doc|doc]] [[Media:SEED Quiz4 2009.pdf|pdf]] | ||
| Lab Day #7: pdf [[Media:SEED_transformation.doc|doc]] | | Lab Day #7: Transformation [[Media:SEED_transformation.pdf|pdf]] [[Media:SEED_transformation.doc|doc]] | ||
|- | |- | ||
| [[../Day 8/]] | | [[../Day 8/]] | ||
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* [[../Sequencing|Sequence results]] | * [[../Sequencing|Sequence results]] | ||
* [[../Sequences|Sequences]] | * [[../Sequences|Sequences]] | ||
| Lab Day #8: pdf [[Media:SEED_beta_gal.doc|doc]] | | Lab Day #8: Beta-Galactosidase: Part I [[Media:SEED_beta_gal.pdf|pdf]] [[Media:SEED_beta_gal.doc|doc]] | ||
|- | |- | ||
| [[../Day 9/]] | | [[../Day 9/]] | ||
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| | | | ||
* Homework #9: TBA | * Homework #9: TBA | ||
| Lab Day #9: pdf [[Media:SEED_inducibile_promoter.doc|doc]] | | Lab Day #9: Beta-Galactosidase: Part II [[Media:SEED_inducibile_promoter.pdf|pdf]] [[Media:SEED_inducibile_promoter.doc|doc]] | ||
|- | |- | ||
| [[../Day 10/]] | | [[../Day 10/]] | ||
Revision as of 13:16, 21 April 2009
http://web.mit.edu/seed/images/seed.gif
[[../Overview/]] of the main lab project
| Class | Date | Agenda | Homework | Lab Protocol |
|---|---|---|---|---|
| [[../Day 1/]] | Feb. 7 | safety training, training lab | Lab Day #1: pdf Serial Dilutions | |
| [[../Day 2/]] | Feb. 21 | SynBio Overview, sterile technique, colony streaking, antibiotic resistance |
|
Lab Day #2: Plate Streaking pdf doc |
| [[../Day 3/]] | Feb. 28 | miniprep, digest | ||
| [[../Day 4/]] | Mar. 7 | PCR and gel | ||
| [[../Day 5/]] | Mar. 14 | gel purification: pdf docx | Lab Day #5: Gel Purification pdf doc | |
| [[../Day 6/]] | Mar. 21 | ligation, transformation |
|
Lab Day #6: Ligation pdf doc |
| [[../Day 7/]] | Mar. 28 | prep for sequencing, glycerol
final project notes:
|
Lab Day #7: Transformation pdf doc | |
| [[../Day 8/]] | Apr. 4 | β-gal assays, growth curves, viable cell titer, analyze sequencing |
|
Lab Day #8: Beta-Galactosidase: Part I pdf doc |
| [[../Day 9/]] | Apr. 18 | device characterization and composability |
|
Lab Day #9: Beta-Galactosidase: Part II pdf doc |
| [[../Day 10/]] | May 2 | data analysis, finish projects |
|
Final Lab: pdf |
