SDS-PAGE

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(Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE))
(SDS-PAGE solutions)
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===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===
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===Running (lower) gel: 10 ml (2 MINIgels)===
 +
 
  Chemical      8%    10%    12%
  Chemical      8%    10%    12%
   
   
Line 5: Line 6:
  30% AA(*)    2.66  3.3    4.0  ml
  30% AA(*)    2.66  3.3    4.0  ml
  LOWER buffer  2.5    2.5    2.5  ml
  LOWER buffer  2.5    2.5    2.5  ml
 +
20% SDS      50    50      50  ul
 +
10% APS      200    200    200  ul
 +
TEMED        8.2    8.2    8.2  ul
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===Stacking (upper) gel: 4 ml (2 MINIgels)===
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 +
Chemical      4%   
 +
 +
Water        2.65 ml
 +
30% AA(*)    0.67 ml
 +
UPPER buffer  0.65 ml
 +
20% SDS      20 ul
 +
10% APS      125 ul
 +
TEMED        6 ul

Revision as of 06:13, 15 May 2007

Running (lower) gel: 10 ml (2 MINIgels)

Chemical      8%     10%     12%

Water         4.74   4.0     3.3  ml
30% AA(*)     2.66   3.3     4.0  ml
LOWER buffer  2.5    2.5     2.5  ml
20% SDS       50     50      50   ul
10% APS       200    200     200  ul
TEMED         8.2    8.2     8.2  ul

Stacking (upper) gel: 4 ml (2 MINIgels)

Chemical      4%     

Water         2.65 ml
30% AA(*)     0.67 ml
UPPER buffer  0.65 ml
20% SDS       20 ul
10% APS       125 ul
TEMED         6 ul
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