SDS-PAGE: Difference between revisions
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30% AA(*) 2.66 3.3 4.0 ml | 30% AA(*) 2.66 3.3 4.0 ml | ||
LOWER buffer 2.5 2.5 2.5 ml | LOWER buffer 2.5 2.5 2.5 ml | ||
20% SDS 50 50 50 ul | 20% [[SDS]] 50 50 50 ul | ||
10% APS 200 200 200 ul | 10% APS 200 200 200 ul | ||
TEMED 8.2 8.2 8.2 ul | TEMED 8.2 8.2 8.2 ul | ||
| Line 17: | Line 17: | ||
30% AA(*) 0.67 ml | 30% AA(*) 0.67 ml | ||
UPPER buffer 0.65 ml | UPPER buffer 0.65 ml | ||
20% SDS 20 ul | 20% [[SDS]] 20 ul | ||
10% APS 125 ul | 10% APS 125 ul | ||
TEMED 6 ul | TEMED 6 ul | ||
| Line 29: | Line 29: | ||
AA = acrylamide | AA = acrylamide | ||
SDS = sodium dodecylsulfate | [[SDS]] = sodium dodecylsulfate | ||
APS = | APS = ammonium persulfate | ||
== Resources== | |||
[http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips. | |||
* [[Lidstrom: SDS-PAGE]] | |||
* [http://webcache.googleusercontent.com/search?q=cache:SWfzFRUgWxUJ:https://www.lablife.org/g?a%3Divy_file%26fh%3DYXvc5936lyS6GTqz6vTO1JNeQgY-%26v%3D1%26id%3Dg2441.CgrzyhkmAUhlRXEHPWLRnldxQuk-%26f%3Dattachments+&cd=7&hl=en&ct=clnk&gl=us protein gel electrophoresis tips and troubleshooting guide] | |||
[[Category:Material]] [[Category:PAGE]] | |||
Latest revision as of 14:09, 2 March 2014
Running (lower) gel: 10 ml (2 MINIgels)
Chemical 8% 10% 12% Water 4.74 4.0 3.3 ml 30% AA(*) 2.66 3.3 4.0 ml LOWER buffer 2.5 2.5 2.5 ml 20% SDS 50 50 50 ul 10% APS 200 200 200 ul TEMED 8.2 8.2 8.2 ul
Stacking (upper) gel: 4 ml (2 MINIgels)
Chemical 4% Water 2.65 ml 30% AA(*) 0.67 ml UPPER buffer 0.65 ml 20% SDS 20 ul 10% APS 125 ul TEMED 6 ul
Buffers
LOWER buffer: 1M Tris base, pH 8.8 UPPER buffer: 1M Tris base, pH 6.8
Abbreviations
AA = acrylamide SDS = sodium dodecylsulfate APS = ammonium persulfate
Resources
SDS-PAGE "Hall of Shame" - examples of common failures with debugging tips.
