SDS-PAGE

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Stacking (upper) gel: 4 ml (2 MINIgels))
(SDS link)
(4 intermediate revisions not shown.)
Line 6: Line 6:
  30% AA(*)    2.66  3.3    4.0  ml
  30% AA(*)    2.66  3.3    4.0  ml
  LOWER buffer  2.5    2.5    2.5  ml
  LOWER buffer  2.5    2.5    2.5  ml
-
  20% SDS      50    50      50  ul
+
  20% [[SDS]]       50    50      50  ul
  10% APS      200    200    200  ul
  10% APS      200    200    200  ul
  TEMED        8.2    8.2    8.2  ul
  TEMED        8.2    8.2    8.2  ul
Line 17: Line 17:
  30% AA(*)    0.67 ml
  30% AA(*)    0.67 ml
  UPPER buffer  0.65 ml
  UPPER buffer  0.65 ml
-
  20% SDS      20 ul
+
  20% [[SDS]]       20 ul
  10% APS      125 ul
  10% APS      125 ul
  TEMED        6 ul
  TEMED        6 ul
Line 29: Line 29:
  AA = acrylamide
  AA = acrylamide
-
  SDS = sodium dodecylsulfate
+
  [[SDS]] = sodium dodecylsulfate
-
  APS = amonium persulfate
+
  APS = ammonium persulfate
 +
 
 +
==Notes==
 +
[http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips.
 +
 
 +
[[Category:Material]] [[Category:PAGE]]

Revision as of 09:48, 8 April 2009

Contents

Running (lower) gel: 10 ml (2 MINIgels)

Chemical      8%     10%     12%

Water         4.74   4.0     3.3  ml
30% AA(*)     2.66   3.3     4.0  ml
LOWER buffer  2.5    2.5     2.5  ml
20% SDS       50     50      50   ul
10% APS       200    200     200  ul
TEMED         8.2    8.2     8.2  ul

Stacking (upper) gel: 4 ml (2 MINIgels)

Chemical      4%     

Water         2.65 ml
30% AA(*)     0.67 ml
UPPER buffer  0.65 ml
20% SDS       20 ul
10% APS       125 ul
TEMED         6 ul

Buffers

LOWER buffer: 1M Tris base, pH 8.8
UPPER buffer: 1M Tris base, pH 6.8

Abbreviations

AA = acrylamide
SDS = sodium dodecylsulfate
APS = ammonium persulfate

Notes

SDS-PAGE "Hall of Shame" - examples of common failures with debugging tips.

Personal tools