SDS-PAGE
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(Difference between revisions)
(→Stacking (upper) gel: 4 ml (2 MINIgels)) |
Current revision (10:48, 8 April 2009) (view source) (SDS link) |
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30% AA(*) 2.66 3.3 4.0 ml | 30% AA(*) 2.66 3.3 4.0 ml | ||
LOWER buffer 2.5 2.5 2.5 ml | LOWER buffer 2.5 2.5 2.5 ml | ||
| - | 20% SDS 50 50 50 ul | + | 20% [[SDS]] 50 50 50 ul |
10% APS 200 200 200 ul | 10% APS 200 200 200 ul | ||
TEMED 8.2 8.2 8.2 ul | TEMED 8.2 8.2 8.2 ul | ||
| Line 17: | Line 17: | ||
30% AA(*) 0.67 ml | 30% AA(*) 0.67 ml | ||
UPPER buffer 0.65 ml | UPPER buffer 0.65 ml | ||
| - | 20% SDS 20 ul | + | 20% [[SDS]] 20 ul |
10% APS 125 ul | 10% APS 125 ul | ||
TEMED 6 ul | TEMED 6 ul | ||
| Line 29: | Line 29: | ||
AA = acrylamide | AA = acrylamide | ||
| - | SDS = sodium dodecylsulfate | + | [[SDS]] = sodium dodecylsulfate |
| - | APS = | + | APS = ammonium persulfate |
| + | |||
| + | ==Notes== | ||
| + | [http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips. | ||
| + | |||
| + | [[Category:Material]] [[Category:PAGE]] | ||
Current revision
Contents |
Running (lower) gel: 10 ml (2 MINIgels)
Chemical 8% 10% 12% Water 4.74 4.0 3.3 ml 30% AA(*) 2.66 3.3 4.0 ml LOWER buffer 2.5 2.5 2.5 ml 20% SDS 50 50 50 ul 10% APS 200 200 200 ul TEMED 8.2 8.2 8.2 ul
Stacking (upper) gel: 4 ml (2 MINIgels)
Chemical 4% Water 2.65 ml 30% AA(*) 0.67 ml UPPER buffer 0.65 ml 20% SDS 20 ul 10% APS 125 ul TEMED 6 ul
Buffers
LOWER buffer: 1M Tris base, pH 8.8 UPPER buffer: 1M Tris base, pH 6.8
Abbreviations
AA = acrylamide SDS = sodium dodecylsulfate APS = ammonium persulfate
Notes
SDS-PAGE "Hall of Shame" - examples of common failures with debugging tips.
