SDS-PAGE

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(SDS-PAGE solutions)
(Stacking (upper) gel: 4 ml (2 MINIgels))
Line 20: Line 20:
  10% APS      125 ul
  10% APS      125 ul
  TEMED        6 ul
  TEMED        6 ul
 +
 +
===Buffers===
 +
 +
LOWER buffer: 1M Tris base, pH 8.8
 +
UPPER buffer: 1M Tris base, pH 6.8
 +
 +
===Abbreviations===
 +
 +
AA = acrylamide
 +
SDS = sodium dodecylsulfate
 +
APS = amonium persulfate

Revision as of 06:17, 15 May 2007

Contents

Running (lower) gel: 10 ml (2 MINIgels)

Chemical      8%     10%     12%

Water         4.74   4.0     3.3  ml
30% AA(*)     2.66   3.3     4.0  ml
LOWER buffer  2.5    2.5     2.5  ml
20% SDS       50     50      50   ul
10% APS       200    200     200  ul
TEMED         8.2    8.2     8.2  ul

Stacking (upper) gel: 4 ml (2 MINIgels)

Chemical      4%     

Water         2.65 ml
30% AA(*)     0.67 ml
UPPER buffer  0.65 ml
20% SDS       20 ul
10% APS       125 ul
TEMED         6 ul

Buffers

LOWER buffer: 1M Tris base, pH 8.8
UPPER buffer: 1M Tris base, pH 6.8

Abbreviations

AA = acrylamide
SDS = sodium dodecylsulfate
APS = amonium persulfate
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