SDS-PAGE
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(Difference between revisions)
(→Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)) |
(→SDS-PAGE solutions) |
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| - | === | + | ===Running (lower) gel: 10 ml (2 MINIgels)=== |
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Chemical 8% 10% 12% | Chemical 8% 10% 12% | ||
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30% AA(*) 2.66 3.3 4.0 ml | 30% AA(*) 2.66 3.3 4.0 ml | ||
LOWER buffer 2.5 2.5 2.5 ml | LOWER buffer 2.5 2.5 2.5 ml | ||
| + | 20% SDS 50 50 50 ul | ||
| + | 10% APS 200 200 200 ul | ||
| + | TEMED 8.2 8.2 8.2 ul | ||
| + | |||
| + | ===Stacking (upper) gel: 4 ml (2 MINIgels)=== | ||
| + | |||
| + | Chemical 4% | ||
| + | |||
| + | Water 2.65 ml | ||
| + | 30% AA(*) 0.67 ml | ||
| + | UPPER buffer 0.65 ml | ||
| + | 20% SDS 20 ul | ||
| + | 10% APS 125 ul | ||
| + | TEMED 6 ul | ||
Revision as of 06:13, 15 May 2007
Running (lower) gel: 10 ml (2 MINIgels)
Chemical 8% 10% 12% Water 4.74 4.0 3.3 ml 30% AA(*) 2.66 3.3 4.0 ml LOWER buffer 2.5 2.5 2.5 ml 20% SDS 50 50 50 ul 10% APS 200 200 200 ul TEMED 8.2 8.2 8.2 ul
Stacking (upper) gel: 4 ml (2 MINIgels)
Chemical 4% Water 2.65 ml 30% AA(*) 0.67 ml UPPER buffer 0.65 ml 20% SDS 20 ul 10% APS 125 ul TEMED 6 ul
