SDS-PAGE: Difference between revisions
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APS = ammonium persulfate | APS = ammonium persulfate | ||
== | == Resources== | ||
[http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips. | [http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips. | ||
* [[Lidstrom: SDS-PAGE]] | |||
[[Category:Material]] [[Category:PAGE | * [https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&ved=0CGMQFjAG&url=https%3A%2F%2Fwww.lablife.org%2Fg%3Fa%3Divy_file%26fh%3DYXvc5936lyS6GTqz6vTO1JNeQgY-%26v%3D1%26id%3Dg2441.CgrzyhkmAUhlRXEHPWLRnldxQuk-%26f%3Dattachments&ei=bZoTU_zAHpGHogTHr4CQDA&usg=AFQjCNGr_gkC6ikFzk-PYro46An3LpyWKg&sig2=5TEB4AQcTiTtr5_act0cfA&bvm=bv.61965928,d.cGUhttps://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&ved=0CGMQFjAG&url=https%3A%2F%2Fwww.lablife.org%2Fg%3Fa%3Divy_file%26fh%3DYXvc5936lyS6GTqz6vTO1JNeQgY-%26v%3D1%26id%3Dg2441.CgrzyhkmAUhlRXEHPWLRnldxQuk-%26f%3Dattachments&ei=bZoTU_zAHpGHogTHr4CQDA&usg=AFQjCNGr_gkC6ikFzk-PYro46An3LpyWKg&sig2=5TEB4AQcTiTtr5_act0cfA&bvm=bv.61965928,d.cGU protein gel electrophoresis tips and troubleshooting guide (download)]] | ||
[[Category:Material]] [[Category:PAGE] |
Revision as of 14:04, 2 March 2014
Running (lower) gel: 10 ml (2 MINIgels)
Chemical 8% 10% 12% Water 4.74 4.0 3.3 ml 30% AA(*) 2.66 3.3 4.0 ml LOWER buffer 2.5 2.5 2.5 ml 20% SDS 50 50 50 ul 10% APS 200 200 200 ul TEMED 8.2 8.2 8.2 ul
Stacking (upper) gel: 4 ml (2 MINIgels)
Chemical 4% Water 2.65 ml 30% AA(*) 0.67 ml UPPER buffer 0.65 ml 20% SDS 20 ul 10% APS 125 ul TEMED 6 ul
Buffers
LOWER buffer: 1M Tris base, pH 8.8 UPPER buffer: 1M Tris base, pH 6.8
Abbreviations
AA = acrylamide SDS = sodium dodecylsulfate APS = ammonium persulfate
Resources
SDS-PAGE "Hall of Shame" - examples of common failures with debugging tips.