SDS - Revision history

Jakob Suckale: /* See also */

Jakob Suckale — Wed, 29 Jul 2009 20:05:52 GMT

Jakob Suckale: categories

Jakob Suckale — Mon, 20 Apr 2009 10:31:25 GMT

Jakob Suckale: categories

Jakob Suckale — Mon, 20 Apr 2009 10:27:13 GMT

Jakob Suckale: illustration

Jakob Suckale — Wed, 08 Apr 2009 15:51:26 GMT

illustration

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		+	[[Image:Sodium dodecyl sulfate.png\|250px\|right\|thumb\|chemical structure of SDS (sodium dodecyl sulphate)]]
		+
	'''SDS (sodium dodecyl sulfate/sulphate)''' is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation.		'''SDS (sodium dodecyl sulfate/sulphate)''' is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation.

Jakob Suckale: /* Critical micelle concentration CMC of SDS */

Jakob Suckale — Wed, 08 Apr 2009 15:44:10 GMT

Critical micelle concentration CMC of SDS

←Older revision		Revision as of 15:44, 8 April 2009
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	== Critical micelle concentration CMC of SDS ==		== Critical micelle concentration CMC of SDS ==
-	CMC of SDS varies with pH. It is about 0.01 mol/L ~~(M) for~~ pH 5-10. At more acidic pH the CMC drops off to about 0.~~07M~~ at pH 2 (Rahman Brown '83 [http://www3.interscience.wiley.com/cgi-bin/fulltext/104026844/PDFSTART]).	+	CMC of SDS varies with pH. It is about 0.009 mol/L at pH 5-10. At more acidic pH the CMC drops off to about 0.007M at pH 2 (Rahman Brown '83 [http://www3.interscience.wiley.com/cgi-bin/fulltext/104026844/PDFSTART]).

	== Stability & Storage ==		== Stability & Storage ==

Jakob Suckale: /* Chemical properties */

Jakob Suckale — Wed, 08 Apr 2009 15:37:27 GMT

Chemical properties

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	* water solubility: 250g/l at 20ºC		* water solubility: 250g/l at 20ºC
-		+	* molecular weight MW: 288.38 g/mol

	== SDS solubilisation of biomolecules ==		== SDS solubilisation of biomolecules ==

Jakob Suckale: see also

Jakob Suckale — Wed, 08 Apr 2009 15:09:06 GMT

Jakob Suckale: /* Chemical properties */

Jakob Suckale — Wed, 08 Apr 2009 15:01:58 GMT

Chemical properties

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	== Chemical properties ==		== Chemical properties ==

		+	* water solubility: 250g/l at 20ºC

	== SDS solubilisation of biomolecules ==		== SDS solubilisation of biomolecules ==

Jakob Suckale: chemical properties

Jakob Suckale — Wed, 08 Apr 2009 15:00:22 GMT

chemical properties

←Older revision		Revision as of 15:00, 8 April 2009
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	see [http://msds.chem.ox.ac.uk/SO/sodium_dodecyl_sulfate.html MSDS]		see [http://msds.chem.ox.ac.uk/SO/sodium_dodecyl_sulfate.html MSDS]

-	== SDS solubilisation ==	+	== Chemical properties ==
		+
		+
		+	== SDS solubilisation of biomolecules ==
	As a rule of thumb for the solubilisation/solubilization of proteins, SDS should be used at its critical micelle concentration (Womack'83 PMID 6882760). For the effects of SDS on protein conformation see Protein Structure by Creighton [http://books.google.de/books?id=KE-WlxzOxF0C&pg=PA349&lpg=PA349&dq=Protein+Structure:+A+Practical+Approach&source=bl&ots=G97vF5AaQ6&sig=m8YQBXGv8_wVKCs9_yr2wuJfqRc&hl=en&ei=VrTcSYLCM4SPsAan-LSmDA&sa=X&oi=book_result&ct=result]. For comparisons between SDS and other detergents for solubilisation of lipids, proteins, and its effect on		As a rule of thumb for the solubilisation/solubilization of proteins, SDS should be used at its critical micelle concentration (Womack'83 PMID 6882760). For the effects of SDS on protein conformation see Protein Structure by Creighton [http://books.google.de/books?id=KE-WlxzOxF0C&pg=PA349&lpg=PA349&dq=Protein+Structure:+A+Practical+Approach&source=bl&ots=G97vF5AaQ6&sig=m8YQBXGv8_wVKCs9_yr2wuJfqRc&hl=en&ei=VrTcSYLCM4SPsAan-LSmDA&sa=X&oi=book_result&ct=result]. For comparisons between SDS and other detergents for solubilisation of lipids, proteins, and its effect on
	enzymes activity see Womack '83 (PMID 6882760) and Creighton Protein Structure.		enzymes activity see Womack '83 (PMID 6882760) and Creighton Protein Structure.

Jakob Suckale: warnings

Jakob Suckale — Wed, 08 Apr 2009 14:59:28 GMT

warnings

←Older revision		Revision as of 14:59, 8 April 2009
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	'''SDS (sodium dodecyl sulfate/sulphate)''' is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation.		'''SDS (sodium dodecyl sulfate/sulphate)''' is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation.

		+	== Warnings ==
		+	* highly flammable
		+	* harmful in contact with skin and if swallowed
		+	* irritating to eyes, respiratory system and skin
		+	see [http://msds.chem.ox.ac.uk/SO/sodium_dodecyl_sulfate.html MSDS]

	== SDS solubilisation ==		== SDS solubilisation ==

←Older revision		Revision as of 20:05, 29 July 2009
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	* [http://www.moleculeoftheday.com/2006/05/04/sodium-dodecyl-sulfate-for-that-fresh-no-scum-feeling/ SDS at molecule of the day blog]		* [http://www.moleculeoftheday.com/2006/05/04/sodium-dodecyl-sulfate-for-that-fresh-no-scum-feeling/ SDS at molecule of the day blog]
	* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=L6026\|SIAL&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC SDS >99% pure at Sigma]		* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=L6026\|SIAL&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC SDS >99% pure at Sigma]
		+	* [[Critical micelle concentration (CMC)]]

	[[Category:Material]][[Category:Detergents]]		[[Category:Material]][[Category:Detergents]]

←Older revision		Revision as of 10:31, 20 April 2009
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	* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=L6026\|SIAL&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC SDS >99% pure at Sigma]		* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=L6026\|SIAL&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC SDS >99% pure at Sigma]

-	[[Category:Material]]	+	[[Category:Material]][[Category:Detergents]]

←Older revision		Revision as of 10:27, 20 April 2009
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	* [http://www.moleculeoftheday.com/2006/05/04/sodium-dodecyl-sulfate-for-that-fresh-no-scum-feeling/ SDS at molecule of the day blog]		* [http://www.moleculeoftheday.com/2006/05/04/sodium-dodecyl-sulfate-for-that-fresh-no-scum-feeling/ SDS at molecule of the day blog]
	* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=L6026\|SIAL&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC SDS >99% pure at Sigma]		* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=L6026\|SIAL&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC SDS >99% pure at Sigma]
		+
		+	[[Category:Material]]

←Older revision		Revision as of 15:09, 8 April 2009
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	* water solubility: 250g/l at 20ºC		* water solubility: 250g/l at 20ºC
		+

	== SDS solubilisation of biomolecules ==		== SDS solubilisation of biomolecules ==
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	Use an ion retardation columns to remove SDS from protein samples.		Use an ion retardation columns to remove SDS from protein samples.
	Retardation Resin. For SDS removal methods see Kapp'78 (PMID 9762103) and similar papers [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=link&linkname=pubmed_pubmed_reviews&uid=9762103&ordinalpos=1&log$=relatedarticles&logdbfrom=pubmed]. Methylene blue can be used to determine the remaining amount of SDS.		Retardation Resin. For SDS removal methods see Kapp'78 (PMID 9762103) and similar papers [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=link&linkname=pubmed_pubmed_reviews&uid=9762103&ordinalpos=1&log$=relatedarticles&logdbfrom=pubmed]. Methylene blue can be used to determine the remaining amount of SDS.
		+
		+	== See also ==
		+	* [http://en.wikipedia.org/wiki/Sodium_lauryl_sulfate SDS at the Wikipedia]
		+	* [http://www.moleculeoftheday.com/2006/05/04/sodium-dodecyl-sulfate-for-that-fresh-no-scum-feeling/ SDS at molecule of the day blog]
		+	* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=L6026\|SIAL&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC SDS >99% pure at Sigma]