Torsten Waldminghaus: add tags

Torsten Waldminghaus — Tue, 11 Mar 2008 15:41:30 GMT

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←Older revision		Revision as of 15:41, 11 March 2008
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	or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast]		or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast]
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		+	[[Category:Material]] [[Category:Defined Media]]

Jgritton at 20:37, 6 June 2005

Jgritton — Mon, 06 Jun 2005 20:37:47 GMT

New page

== Summary ==
Synthetic Dextrose (SD) Medium. Defined Minimal yeast medium
== Ingredients ==
Carbon source:
::Dextrose 20g/L
Nitrogen source:
::Ammonium sulfate 5g/L
Vitamins:
::Biotin 20μg/L
::Calcium pantothenate 2mg/L
::Folic acid 2μg/L
::Inositol 10mg/L
::Niacin 400μg/L
::p-Aminobenzoic acid 200μg/L
::Pyridoxine hydrochloride 400μg/L
::Riboflavin 200μg/L
::Thiamine hydrochloride 400μg/L
Compounds supplying trace elements:
::Boric acid 500μg/L
::Copper sulphate 40μg/L
::Potassium iodide 100μg/L
::Ferric chloride 200μg/L
::Manganese sulphate 400μg/L
::Sodium molybdate 200μg/L
::Zinc sulphate 400μg/L
Salts:
::Potassium phosphate monobasic 850mg/L
::Potassium phosphate dibasic 150mg/L
::Magnesium sulphate 500mg/L
::Sodium chloride 100mg/L
::Calcium chloride 100mg/L
==Protocol==
# Mix 6.7 g Difco YNB w/o Amino Acids with distilled water to 950 mL
#*If making plates add 20 g Agar in addition to YNB
#Autoclave (liquid cycle)
#* 250° F, 22 psi, 30 minutes
#Add 50 mL 40% glucose (available from the kitchen)

==Source==
Adapted From:

F. Sherman, ''Getting started with yeast'', Methods Enzymol. 350, 3-41 (2002)

or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast]

SD - Revision history

Torsten Waldminghaus: add tags

Jgritton at 20:37, 6 June 2005