SD
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Summary
Synthetic Dextrose (SD) Medium. Defined Minimal yeast medium
Ingredients
Carbon source:
- Dextrose 20g/L
Nitrogen source:
- Ammonium sulfate 5g/L
Vitamins:
- Biotin 20μg/L
- Calcium pantothenate 2mg/L
- Folic acid 2μg/L
- Inositol 10mg/L
- Niacin 400μg/L
- p-Aminobenzoic acid 200μg/L
- Pyridoxine hydrochloride 400μg/L
- Riboflavin 200μg/L
- Thiamine hydrochloride 400μg/L
Compounds supplying trace elements:
- Boric acid 500μg/L
- Copper sulphate 40μg/L
- Potassium iodide 100μg/L
- Ferric chloride 200μg/L
- Manganese sulphate 400μg/L
- Sodium molybdate 200μg/L
- Zinc sulphate 400μg/L
Salts:
- Potassium phosphate monobasic 850mg/L
- Potassium phosphate dibasic 150mg/L
- Magnesium sulphate 500mg/L
- Sodium chloride 100mg/L
- Calcium chloride 100mg/L
Protocol
- Mix 6.7 g Difco YNB w/o Amino Acids with distilled water to 950 mL
- If making plates add 20 g Agar in addition to YNB
- Autoclave (liquid cycle)
- 250° F, 22 psi, 30 minutes
- Add 50 mL 40% glucose (available from the kitchen)
Source
Adapted From:
F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002)
or as a pdf: Getting started with yeast