SC

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SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.
SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.
==Protocol==
==Protocol==
-
Dropout powders can be purchased from [http://www.qbiogene.com/ Qbiogene] and used to make 10X stock solutions. These solutions can be added to [[SD]] after autoclaving.
+
Dropout powders can be purchased from [http://www.qbiogene.com/ Qbiogene] or from [http://www.formedium.com/ Formedium] and used to make 10X stock solutions. These solutions can be added to [[SD]] after autoclaving.
==Source==
==Source==

Revision as of 11:02, 8 October 2006

Contents

Summary

Synthetic Complete (SC) Medium aka (CSM, CM). Defined yeast medium

Ingredients

SD Media with one or more of the following supplements:

Adenine sulfate 20 mg/L
Uracil 20 mg/L
L-Tryptophan 20 mg/L
L-Histidine-HC1 20 mg/L
L-Arginine-HC1 20 mg/L
L-Methionine 20 mg/L
L-Tyrosine 30 mg/L
L-Leucine 60 mg/L
L-Isoleucine 30 mg/L
L-Lysine-HC1 30 mg/L
L-Phenylalanine 50 mg/L
L-Glutamic acid 100 mg/L
L-Aspartic acid 100 mg/L
L-Valine 150 mg/L
L-Threonine 200 mg/L
L-Serine 400 mg/L

SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.

Protocol

Dropout powders can be purchased from Qbiogene or from Formedium and used to make 10X stock solutions. These solutions can be added to SD after autoclaving.

Source

Adapted From:

F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002)

or as a pdf: Getting started with yeast

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