SC: Difference between revisions
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or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast] | or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast] | ||
[[Category:Material]] [[Category:Yeast]] | |||
Revision as of 11:12, 15 February 2008
Summary
Synthetic Complete (SC) Medium aka (CSM, CM). Defined yeast medium
Ingredients
SD Media with one or more of the following supplements:
- Adenine sulfate 20 mg/L
- Uracil 20 mg/L
- L-Tryptophan 20 mg/L
- L-Histidine-HC1 20 mg/L
- L-Arginine-HC1 20 mg/L
- L-Methionine 20 mg/L
- L-Tyrosine 30 mg/L
- L-Leucine 60 mg/L
- L-Isoleucine 30 mg/L
- L-Lysine-HC1 30 mg/L
- L-Phenylalanine 50 mg/L
- L-Glutamic acid 100 mg/L
- L-Aspartic acid 100 mg/L
- L-Valine 150 mg/L
- L-Threonine 200 mg/L
- L-Serine 400 mg/L
SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.
Protocol
Dropout powders can be purchased from Qbiogene or from Formedium and used to make 10X stock solutions. These solutions can be added to SD after autoclaving.
Source
Adapted From:
F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002)
or as a pdf: Getting started with yeast
