SBB13 gels

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(JCAnderson 11:26, 13 March 2013 (EDT))
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[[Image:2013_03_13-JCA-gel1.jpg | 200px ]]<br>
[[Image:2013_03_13-JCA-gel1.jpg | 200px ]]<br>
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This gel is of the PCA2 products for the various synthons.  The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels).  All but 3 look fine.  Lanes 2 and 6 look recoverable with a second PCR.  The lane 1 reaction needs to be started over from scratch.  Lane 5 just needs a careful gel purification.
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This gel is of the PCA2 products for the various synthons.  The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels).  All but three look fine.  Lanes 2 and 6 look recoverable with a second PCR.  The lane 1 reaction needs to be started over from scratch.  Lane 5 just needs a careful gel purification.

Revision as of 11:31, 13 March 2013

JCAnderson 11:26, 13 March 2013 (EDT)


This gel is of the PCA2 products for the various synthons. The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels). All but three look fine. Lanes 2 and 6 look recoverable with a second PCR. The lane 1 reaction needs to be started over from scratch. Lane 5 just needs a careful gel purification.

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