SBB13 gels: Difference between revisions
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==[[User:JCAnderson|JCAnderson]] 11:26, 13 March 2013 (EDT)== | ==[[User:JCAnderson|JCAnderson]] 11:26, 13 March 2013 (EDT)== | ||
This gel is of the PCA2 products for the various synthons. The first lane is the His-tag part (currently unnamed), then molecular weight standards, then the rest are | [[Image:2013_03_13-JCA-gel1.jpg | 200px ]]<br> | ||
This gel is of the PCA2 products for the various synthons. The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels). All but three look fine. Lanes 4 and 8 look recoverable with a second PCR. The lane 3's reaction needs to be started over from scratch. Lane 7 just needs a careful gel purification. | |||
Latest revision as of 08:33, 13 March 2013
JCAnderson 11:26, 13 March 2013 (EDT)
This gel is of the PCA2 products for the various synthons. The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels). All but three look fine. Lanes 4 and 8 look recoverable with a second PCR. The lane 3's reaction needs to be started over from scratch. Lane 7 just needs a careful gel purification.
