SBB13Ntbk - Mar12: Difference between revisions
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(New page: ==Regular Zymo Cleanup== #Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction. #Transfer into the Zymo column (small clear guys) #spin through (top speed, 30 sec), dis...) |
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Revision as of 10:03, 12 March 2013
Regular Zymo Cleanup
- Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction.
- Transfer into the Zymo column (small clear guys)
- spin through (top speed, 30 sec), discard waste.
- Add 200 uL of Zymo Wash Buffer (which is basically 70% ethanol)
- spin through, discard waste.
- Add 200 uL of Zymo Wash Buffer
- spin through, discard waste.
- spin for 90 seconds, full speed to dry.
- elute with water into a fresh Eppendorf tube, use the same volume of water as the volume of the original reaction