SBB13Ntbk-Harini Sadeeshkumar: Difference between revisions
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== | ==Vanillin - quiC-3== | ||
<pre> | <pre> | ||
PCA quiC-3_oligo_1-16 (460 bp, pca1) | PCA quiC-3_oligo_1-16 (460 bp, pca1) | ||
Line 6: | Line 6: | ||
(get pre-digested vector from JCA of pBca9145-Bca1144 (EcoRI/BamHI, vect_dig) | (get pre-digested vector from JCA of pBca9145-Bca1144 (EcoRI/BamHI, vect_dig) | ||
Ligate pcr_dig and vect_dig (pBca9145-quiC-3) | Ligate pcr_dig and vect_dig (pBca9145-quiC-3) | ||
------------------------------- | |||
<quiC-3_oligo_1 | |||
TGATCTAATTGCAATTCCAATCCTGTTTCCATGAACCTTATTTGAATCGAA | |||
<quiC-3_oligo_2 | |||
TCGTAGGTAATAAGTTCGAGAACTATTCTGACGGTTTGCAAATCAATTGGG | |||
<quiC-3_oligo_3 | |||
TCCTTCGTGTTCCAAAGCTGGAACACCGAAAACGATTGCACCTAATCTTTG | |||
<quiC-4_oligo_4 | |||
TCAATTTAACAGGAGATGGTAACATTTTCGATTCAAATAAGGTTCATGGAA | |||
<quiC-5_oligo_5 | |||
GTGTTCCAGCTTTGGAACACGAAGGATTTGTAGGTTCTATGAGACGGCATG | |||
<quiC-6_oligo_6 | |||
GTAAAAGAAACTACATTGCTTATAATGAATTGACAAATAACTCTTTGGGTT | |||
<quiC-7_oligo_7 | |||
CATTAACTAAGAACCAAATTTGGGATAATGGAAAGGACATCAAGAGGTGTG | |||
<quiC-8_oligo_8 | |||
ATTTGGAACACAAGAACCTCCAGCTTCACACCTCTTGATGTCCTTTCCATT | |||
<quiC-9_oligo_9 | |||
ATTATAAGCAATGTAGTTTCTTTTACCCCAATTGATTTGCAAACCGTCAGA | |||
<quiC-10_oligo_10 | |||
ATCCCAAATTTGGTTCTTAGTTAATGTGATTCTTGCATTAGCATCCTTTTC | |||
<quiC-11_oligo_11 | |||
ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCAAATGCTG | |||
<quiC-12_oligo_12 | |||
ATAGTTCTCGAACTTATTACCTACGACAGCATTTGAGACGATGCAGATCTCA | |||
<quiC-13_oligo_13 | |||
ACAGGATTGGAATTGCAATTAGATCAGAAAAGGATGCTAATGCAAGAATCA | |||
<quiC-14_oligo_14 | |||
AATGTTACCATCTCCTGTTAAATTGAAACCCAAAGAGTTATTTGTCAATTC | |||
<quiC-15_oligo_15 | |||
AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCATAGAACCTACAAA | |||
<quiC-16_oligo_16 | |||
AAGCTGGAGGTTCTTGTGTTCCAAATCAAAGATTAGGTGCAATCGTTTTCG | |||
</pre> | </pre> |
Revision as of 23:41, 5 March 2013
Vanillin - quiC-3
PCA quiC-3_oligo_1-16 (460 bp, pca1) PCR pca1 with quiC-3_oligo_11/quiC-3_oligo_15 (460 bp, pca2) Digest pca2, gp (EcoRI/BamHI, 20+415+25 bp, pcr_dig) (get pre-digested vector from JCA of pBca9145-Bca1144 (EcoRI/BamHI, vect_dig) Ligate pcr_dig and vect_dig (pBca9145-quiC-3) ------------------------------- <quiC-3_oligo_1 TGATCTAATTGCAATTCCAATCCTGTTTCCATGAACCTTATTTGAATCGAA <quiC-3_oligo_2 TCGTAGGTAATAAGTTCGAGAACTATTCTGACGGTTTGCAAATCAATTGGG <quiC-3_oligo_3 TCCTTCGTGTTCCAAAGCTGGAACACCGAAAACGATTGCACCTAATCTTTG <quiC-4_oligo_4 TCAATTTAACAGGAGATGGTAACATTTTCGATTCAAATAAGGTTCATGGAA <quiC-5_oligo_5 GTGTTCCAGCTTTGGAACACGAAGGATTTGTAGGTTCTATGAGACGGCATG <quiC-6_oligo_6 GTAAAAGAAACTACATTGCTTATAATGAATTGACAAATAACTCTTTGGGTT <quiC-7_oligo_7 CATTAACTAAGAACCAAATTTGGGATAATGGAAAGGACATCAAGAGGTGTG <quiC-8_oligo_8 ATTTGGAACACAAGAACCTCCAGCTTCACACCTCTTGATGTCCTTTCCATT <quiC-9_oligo_9 ATTATAAGCAATGTAGTTTCTTTTACCCCAATTGATTTGCAAACCGTCAGA <quiC-10_oligo_10 ATCCCAAATTTGGTTCTTAGTTAATGTGATTCTTGCATTAGCATCCTTTTC <quiC-11_oligo_11 ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCAAATGCTG <quiC-12_oligo_12 ATAGTTCTCGAACTTATTACCTACGACAGCATTTGAGACGATGCAGATCTCA <quiC-13_oligo_13 ACAGGATTGGAATTGCAATTAGATCAGAAAAGGATGCTAATGCAAGAATCA <quiC-14_oligo_14 AATGTTACCATCTCCTGTTAAATTGAAACCCAAAGAGTTATTTGTCAATTC <quiC-15_oligo_15 AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCATAGAACCTACAAA <quiC-16_oligo_16 AAGCTGGAGGTTCTTGTGTTCCAAATCAAAGATTAGGTGCAATCGTTTTCG